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  • Brassica (cell wall)  (1)
  • Hordeum vulgare  (1)
  • 1
    ISSN: 1432-2048
    Keywords: Key words: Antibody (JIM 5 ; JIM 7) ; Brassica (cell wall) ; Cell wall (structure) ; Epitope (pectin) ; Methyl-esterification ; Pectic polysaccharide
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. Pectic substances are a major component of cell walls in vegetable plants and have an important influence on plant food texture. Cauliflower (Brassica oleracea L. var. botrytis) stem sections at different regions of the mature plant stem have been monitored for tissue-related changes in the native pectic polysaccharides. Chemical analysis detected appreciable differences in the degree of methyl-esterification (ME) of pectic polysaccharides. About 65% of galacturonic acid (GalpA) residues were methyl-esterified in floret tissues. Relative ME showed a basipetal decrease, from 94% in the upper stem to 51% in the lower-stem vascular tissues. The decrease was not related to a basipetal increase in glucuronic acid (GlcpA) residues. The monoclonal antibodies, JIM 5 and JIM 7, produced distinct labelling patterns for the relatively low-methyl-esterified and high-methyl-esterified pectin epitopes, respectively. Labelling was related to cell type and tissue location in the stem. Floret cell walls contained epitopes for both JIM 5 and JIM 7 throughout the wall. Stem vascular tissues labelled more strongly with JIM 5. Whereas pith parenchyma in the upper stem labelled more strongly with JIM 7, in the lower-stem pith parenchyma, JIM 5 labelling predominated. Localization of pectic polysaccharide epitopes in cell walls provides an insight into how structural modifications might relate to the textural and nutritional properties of cell walls.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Biology and fertility of soils 12 (1991), S. 10-18 
    ISSN: 1432-0789
    Keywords: Agroecological rotation ; Hordeum vulgare ; Microbial biomass ; 15N ; Rotation effects ; Pot experiment ; Soil nitrogen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Soil N dynamics and barley yields (Hordeum vulgare L.) were compared in pot experiments using surface samples from a Gray Luvisol under three cropping systems at Breton, Alberta: (1) an agroecological 8-year rotation including cereals, forage, and fababeans (Vicia faba L.) as green manure, from wich two plots were selected, one following fababeans, and the second following 3 years of forage; (2) a continuous grain system, with fertilizer N at 90 kg ha-1 year-1; and (3) a classical Breton 5-year rotation [following oats (Avena sativa L.)] involving forage and cereals, without returning crop residues to the land, selecting one plot with PKS treatment and a second as control. The fertilizer N equivalent for the cropping system; “AN” value and “A” value (analogous to AN), but in fertilizer 15N units, soil biomass, and C and N mineralization were monitored. In the first agroecological plot (after fababeans), grain and total plant biomass production were 116% greater than from the continuous grain treatment. Barley plants in the two agroecological plots derived 48.5% and 37.8%, respectively, of their N requirement from non-labelled soil N sources not present in the continuous grain plot. At crop maturity, the recovery of 15N microbial biomass was 1.5 times higher in soil from the first agroecological plot than from the continuous grain plot. The fertilizer N equivalent was 2670 mg pot-1 (485 kg ha-1) for the first and 1850 mg pot-1 for the second agroecological treatment. Fertilizer N equivalent values exceed net amounts of N mineralized by a factor of 4. Recovery by the barley crop of 15N added at 55 mg pot-1 was more efficient in the agroecological treatments (45%–51%) than in the continuous grain or classical Breton treatments (35%–37%). It was concluded (1) that past soil history may be associated more with the ability of barley plants to compete for available N, and hence the use of N, than with net soil N mineralization; and (2) an increased supply of N to crops following the incorporation of fababean residues, manure application, and the soil N-conserving effect of growing legumes were all partly responsible for the observed differences in soil fertility.
    Type of Medium: Electronic Resource
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