ISSN:
1573-5028
Keywords:
Brassica napus
;
DNA-binding proteins
;
light-inducible gene
;
tissue specificity
;
transmembrane protein
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Using a fractionated genomic bank, we have cloned and characterized a Brassica napus gene (rbcSF1) encoding the small sub-unit of ribulose 1,5-bisphosphate carboxylase. The promoter of this gene contains a 29 bp direct repeat capable of forming a single or a double hairpin loop, and three elements that are recognized by leaf nuclear proteins in vitro. The most upstream are the S-box, a small A/T-rich sequence between −516 and −512, and the F-box between −492 and −475. Finally, we have also observed binding to the G-box, a regulatory element common to numerous plant promoters. The promoter of rbcSF1 also has a 113 amino acids open reading frame (ORF113) in the non-coding strand. When used to probe a northern blot of leaf RNA, this ORF hybridizes to a 1.5 kb transcript. The protein encoded by ORF113 contains a transmembrane domain.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00016068
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