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  • 1
    ISSN: 1432-2242
    Keywords: Key words Microsatellites ; Lycopersicon esculentum ; STMS ; Cultivar identification ; Fluorescent detection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The objectives of this study were to evaluate the usefulness of a fluorescent-analysis method for genotyping PCR-based tomato microsatellite markers (or STMSs) and to establish the value of these markers to generate unique DNA profiles of tomato cultivars. The analyses were performed using forward primers labelled with a fluorochrom and using an ALF express DNA sequencer. In general, analysis of the tomato STMSs revealed distinct allelic peaks. PCR artefacts like stuttering and differential amplification were observed for several tomato STMS markers, but in most cases these artefacts did not seriously hamper allele designation. Comparison of fluorescent and silver-stained allelic profiles revealed a similar distribution of alleles among the test cultivars. Sixteen tomato cultivars were DNA-typed for 20 selected STMS markers using the fluorescent approach. Length polymorphism among the PCR products was detected with 18 of these markers, yielding gene diversity values from 0.06 to 0.74. The number of alleles per microsatellite locus ranged from 2 to 8. As few as four STMSs were sufficient to differentiate between all 16 cultivars, indicating that these markers are especially suitable for a species like tomato which has low levels of variation as detected by other types of markers.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-5060
    Keywords: Brassica oleracea ; bud stage ; embryogenesis ; genetic variation ; haploidy ; microspore culture ; regeneration ; tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Microspore culture was shown to be applicable to a broad range of accessions belonging to six horticulturally important crop types of Brassica oleracea: broccoli, white cabbage, cauliflower, savoy cabbage, Brussels sprouts and curly kale. Of 64 accessions tested 86% were responsive. Large genotypic differences were found in number of embryos produced per flower bud, and in frequency and mode of regeneration of plants from embryos. B. oleracea was characterized by a strong asynchrony of microspore development within single buds. Microspore populations optimal for culture contained a large proportion (10–40%) of binucleate pollen. An initial high temperature treatment was essential for microspore embryogenesis. Growth conditions of the donor plants during inflorescence formation were less critical.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Genetic resources and crop evolution 43 (1996), S. 343-349 
    ISSN: 1573-5109
    Keywords: duplication ; bulking ; Brassica oleracea ; Brussels sprouts ; white cabbage ; genetic resources ; isozymes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary To reduce the number of accessions in the Brassica oleracea collection of the Centre for Genetic Resources The Netherlands (CGN) groups of accessions were bulked. Accessions in a group were selections from the same landrace or old variety, and were chosen, with the help of crop experts, on the basis of their history and morphology. Since this approach was received rather sceptically in the genebank community, a genetical analysis of some of the groups and their components (named varieties) was made to justify the procedures. A selection of white cabbage and Brussels sprouts, representing two crop types of B. oleracea, was made: eleven white cabbage groups comprising 43 components, and nine Brussels sprouts groups comprising 24 components. Each group and all of its components were described on the basis of electrophoresis of nine isoenzymes on 30 plants. Most components were rightly classified; 16 percent were misclassified. All these misclassifications were within similar groups. It could be concluded that pooling of the components included in this study was justified. In two cases the isozymic patterns suggested that the groups could even have been larger. Only in the case of the two groups of Brussels sprouts ‘Bedfordshire’ this was a real option. In the other case involving the white cabbage groups ‘Langedijker Bewaar Gewoon’, ‘Langedijker Bewaar Graag’ and ‘Late Herfstdeen’ this was not an option since these landraces, although having a common genetic background, clearly have a distinct identity as defined by morphology and history.
    Type of Medium: Electronic Resource
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