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  • BrdUrd-labelling  (1)
  • Lung cancer  (1)
  • Virusinfekt  (1)
  • 1
    Electronic Resource
    Electronic Resource
    The applications of the BrdUrd-technique for the estimation of cycling S-phase cells in human renal cell carcinoma (1988)
    Springer
    Urological research 16 (1988), S. 303-307 
    Details
    ISSN: 1434-0879
    Keywords: Proliferation of renal cell carcinoma ; Flow cytometry ; BrdUrd-labelling ; S-phase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary After testing the BrdUrd technique on experimental tumour cell lines, we applied the technique to human renal cell carcinoma in vitro. We compared the results with the data acquired after FCM analysis and 3H-thymidine treatment. In contrast to BrdUrd the 3H-thymidine uptake seemed to be limited in suspended cells. FCM data represented the DNA distribution of cells. BrdUrd labelling on the other hand detected DNA synthesizing cells. Only both methods in parallel were able to discriminate between proliferating cells and resting cells with an S-phase equivalent DNA content.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00263640
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Die Impulseytophotometrie in der hämatologischen Cytologie (1971)
    Springer
    Journal of molecular medicine 49 (1971), S. 1090-1092 
    Details
    ISSN: 1432-1440
    Keywords: Pulse cytophotometry ; DNA-content ; cell kinetics ; leukemia ; virus infection ; Impulscytophotometrie ; DNS-Gehalt ; Zellkinetik ; Leukämie ; Virusinfekt
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung In der Impulscytophotometrie bietet sich jetzt eine Methode für die hämatologische Diagnostik, die innerhalb von wenigen Minuten Daten über die Proliferationskinetik, im besonderen über den Anteil der G1-Phasezellen, S-Phasezellen und (G2+M)-Phasezellen an der Gesamtpopulation sowie über aneuploide Stammzellinien schnell und genau liefert.
    Notes: Summary The “impulse cytophotometry” contributes considerably to the haematological diagnosis, producing within a few minutes data about the proliferation kinetics. Quick and correct assessment of G1-phase cells, S-phase cells and (G2+M)-phase cells as well as data on aneuploid cell stemlines is thus facilitated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01732921
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Increased number of cancer cells in bronchial washing fluid detected by combining conventional cytology and high-resolution flow cytometry (1997)
    Springer
    Journal of cancer research and clinical oncology 123 (1997), S. 571-577 
    Details
    ISSN: 1432-1335
    Keywords: Key words DNA/protein flow cytometry ; Bronchial washing fluid ; Cytology ; Early diagnosis ; Lung cancer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The present study was performed to improve early lung cancer diagnosis in bronchial washing fluid, thereby increasing the diagnostic sensitivity of bronchoscopy by means of high-resolution flow cytometry (FC). We combined dual-parameter DNA/protein FC and conventional cytology in bronchial washing fluid samples from 112 patients with neoplastic and non-neoplastic lung diseases and found 43% of histologically confirmed tumor cases to be cytologically positive; 63% of the tumor samples were aneuploid, 52% of the aneuploid cases were cytologically positive and 48% were negative. In the negative cases, FC was an independent diagnostic factor. In 32% of the cases, FC also failed to detect abnormalities. However, the combination of both techniques increased the sensitivity in detecting neoplastic cells to 73%. Furthermore, simultaneous DNA/protein analysis allowed the recognition of aneuploid cell lines not detectable by single DNA measurement. Identification of aneuploid subpopulations by dual-parameter analysis in cytologically negative one-parameter FC “diploid” samples assumes an important diagnostic value. Dual-parameter DNA/protein FC is a valuable technique that increases the diagnostic yield of bronchoscopy with no risk for the patient and a low additional cost.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004320050107
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    Paper (German National Licenses)
    Fulltext
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