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Digitale Medien

Diagnosis of bronchopulmonary infections by quantification of microflora (1994)

Pirali, F. ; Longo, M. ; Gelmi, M. ; [weitere]

Springer

European journal of epidemiology 10 (1994), S. 703-706

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Details

ISSN: 1573-7284

Schlagwort(e): Bronchial microflora ; Bronchopulmonary infections ; Quantification

Quelle: Springer Online Journal Archives 1860-2000

Thema: Medizin

Notizen: Abstract The quantification of bacteria and fungi in sputum or bronchoaspirate is of clinical value for the diagnosis of respiratory tract infections. We have developed an easy method to count the micro-organisms in patients with respiratory tract infections. This consists of the quantification of micro-organisms by subsequent streakings of a calibrated loop on agar. The correlation between microbiological quantitative data and the clinical status of patients with lower respiratory tract infections is discussed. The data seem to indicate that certain bacteria present in sputum or bronchoaspirate above a certain concentration may be responsible for lower respiratory tract infections. In patients with immunological disorders or chronic pathologies even lower concentrations of micro-organisms in bronchial secretions probably are enough to cause infections. The advantage of this counting method of the microbic species from the respiratory tract consists of their quantification: thus we can attribute an etiological role to a high concentration of the germs, while micro-organisms at low concentrations are probably contaminants. By this method isolated colonies are obtained after 12–18 hours. The bacterial quantification, by respiratory samples examination of the same patient in the following days, allows us to evaluate the efficacy of antibacterial therapy, producing a reduction of bacterial concentration.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF01719285

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Digitale Medien

Design and operation of a simple environmental chamber for rapid freezing fixation (1991)

Bailey, S. M. ; Chiruvolu, S. ; Longo, M. L. ; [weitere]

New York, NY : Wiley-Blackwell

Journal of Electron Microscopy Technique 19 (1991), S. 118-126

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Details

ISSN: 0741-0581

Schlagwort(e): Freeze-fracture ; Cryotechniques ; Humidity ; Temperature control ; Life and Medical Sciences ; Cell & Developmental Biology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Allgemeine Naturwissenschaft

Notizen: Rapid freezing is the most important step in sample preparation for freeze-fracture and other cryotechniques for electron microscopy. We present the design and operation of a simple environmental chamber coupled to a plunger-driven freezing device that has provided simple and reliable freezing from temperatures and humidities other than ambient. The chamber can be constructed and operated with equipment and techniques common to most electron microscopy labs. Temperature control of ±0.1°C and relative humidities of 〉90% were provided over the range -5-60°C. Typical electron micrographs showing well preserved structures comparable to jet-freezing are presented.

Zusätzliches Material: 7 Ill.