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  • 1
    Electronic Resource
    Electronic Resource
    Transcriptional control of plastid gene expression in greening Sorghum seedlings (1991)
    Springer
    Planta 183 (1991), S. 101-111 
    Details
    ISSN: 1432-2048
    Keywords: Chloroplast biogenesis ; Plastid gene expression ; Sorghum ; Transcriptional regulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The greening of 5-d-old etiolated Sorghum seedlings was used to investigate regulatory mechanisms of plastid gene expression. Sorghum was selected for this study because leaves of etiolated seedlings do not expand in the dark and are kept within the coleoptile, thus leaf development is inhibited during etiolation. Only traces of thylakoid-membrane polypeptides and plastid transcripts could be detected in dark-grown seedlings, the accumulation of both protein and RNA was found to be strictly dependent upon light. Run-on transcription analysis demonstrated that the rise in plastid RNA levels was due to a light-induced increase in plastid transcriptional activity and not to altered RNA stability. Using an antiserum to the β subunit of plastid RNA polymerase, it could be demonstrated that the increase in plastid transcriptional activity was paralleled by rising levels of plastid RNA polymerase. It has to be concluded, therefore, that in greening Sorghum seedlings the expression of plastid genes is photoregulated at the transcriptional level.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00197573
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Functional anatomy of the mechanoreceptor cells in tendrils of Bryonia dioica Jacq. (1995)
    Springer
    Planta 196 (1995), S. 539-550 
    Details
    ISSN: 1432-2048
    Keywords: Actin ; Bryonia ; Cytoskeleton (tactile blep) ; Tactile blep ; Tendril (ultrastructure) ; Tendril mechano-sensor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The ultrastructure of epidermal cells of tendrils of Bryonia dioica Jacq. (Cucurbitaceae) was determined using microscopic, histochemical and immunochemical techniques with focus on the tactile blep, the mechano-receptor of these cells. Tactile bleps resemble bordered pits in structure and probably in formation. They contain cytoplasm rich in endoplasmic reticulum, dictyosomes, mitochondria and microbodies. The cytoplasm is highly vesiculated and usually contains lipid-body-like structures. Cytoskeletal elements (microtubules, actin filaments) are uniquely arranged in the tactile blep, and chlorotetracycline-fluorescence analysis shows large amounts of membrane-associated calcium within the tactile blep. We propose a physically interconnected cytoskeleton-membrane device as the immediate force sensor and transducer which creates a primary intracellular signal, for which calcium is a likely candidate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00203654
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    Paper (German National Licenses)
    Fulltext
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