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  • 1
    ISSN: 1617-4623
    Keywords: 18S rRNA transcription ; Plant mitochondrial promoters ; Arabidopsis ; Pea ; Potato
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Transcription initiation sites for the mitochondrial 18S rRNA genes in the dicot plantsArabidopsis thaliana, potato and pea were identified by a combination of in vitro capping, primer extension and S-1 analyses. These promoters contain a nonanucleotide motif and an AT-rich sequence similar to many mRNA and tRNA promoters in dicot mitochondria. InArabidopsis and potato, active promoters are located within 120 nucleotides upstream of the 18S rRNA genes, as inOenothera. The nucleotide sequence in the corresponding region in pea mitochondria is well conserved, but is not used as promoter in this plant. Instead a novel promoter sequence is used that lies several hundred nucleotides upstream. These results show that rRNAs can be transcribed from the same promoter types as mRNAs and tRNAs in plant mitochondria. However, the sequence features presently attributed to plant mitochondrial promoters — the conserved nonanucleotide and the upstream AT-rich box — do not allow to deduce the presence of an active promoter from genomic sequence data alone.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-2048
    Keywords: Bryophyllum ; Crown-gall ; 3′:5′-cyclic-AMP phosphodiesterase ; Theophylline
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In crown-gall tumor tissue obtained from leaves of Bryophyllum daigremontianum an adenosine 3′:5′-cyclic phosphate (3′:5′-cyclic-AMP) degrading activity increases up to 2.5 fold until the fifth day after inoculation with Agrobacterium tumefaciens, declining to the value of the control in the solid tumor. Theophylline up to 1 mmol l−1 given to wounded leaves of Bryophyllum daigremontianum has no effect on the number of tumors. The effect of higher concentrations given over extended periods can be explained otherwise. Therefore it seems likely that the 3′:5′-cyclic-AMP phosphodiesterase (EC 3.1.4.17) has no effect on transformation and growth of crown-gall tumors in Bryophyllum daigremontianum.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1617-4623
    Keywords: Key words 18S rRNA transcription ; Plant mitochondrial promoters ; Arabidopsis ; Pea ; Potato
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Transcription initiation sites for the mitochondrial 18S rRNA genes in the dicot plants Arabidopsis thaliana, potato and pea were identified by a combination of in vitro capping, primer extension and S-1 analyses. These promoters contain a nonanucleotide motif and an AT-rich sequence similar to many mRNA and tRNA promoters in dicot mitochondria. In Arabidopsis and potato, active promoters are located within 120 nucleotides upstream of the 18S rRNA genes, as in Oenothera. The nucleotide sequence in the corresponding region in pea mitochondria is well conserved, but is not used as promoter in this plant. Instead a novel promoter sequence is used that lies several hundred nucleotides upstream. These results show that rRNAs can be transcribed from the same promoter types as mRNAs and tRNAs in plant mitochondria. However, the sequence features presently attributed to plant mitochondrial promoters – the conserved nonanucleotide and the upstream AT-rich box – do not allow to deduce the presence of an active promoter from genomic sequence data alone.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
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