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  • Dystrophic axons  (1)
  • Growth hormone  (1)
  • Herpes simplex virus  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Isolation, culture and cell-type identification of adult human pituitary cells (1985)
    Springer
    Acta neuropathologica 68 (1985), S. 205-208 
    Details
    ISSN: 1432-0533
    Keywords: Human pituitary gland ; Tissue culture ; Density gradients ; Immunocytochemistry ; Growth hormone ; Prolactin ; Adrenocorticotropin ; Follicle-stimulating hormone ; Luteinizing hormone ; Thyrotropin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Pituitary cells were isolated from adult human pituitary glands obtained at autopsy 4–12 h postmortem by enzyme treatment (collagenase and dispase) and by Percoll density gradients. Cells thus isolated were maintained in culture for more than 6 months. By immunoperoxidase staining methods using rabbit sera monospecific against various pituitary hormones, a large number of cultured cells reacted positively. The hormones identified in these cells were growth hormone, prolactin, adrenocorticotropin, follicle-stimulting hormone, luteinizing hormone and thyrotropin. Electron-microscopic examination of cultured cells revealed the presence of secretory granules in cytoplasm characteristic of in vivo human pituitary cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00690196
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Morphological alterations of Purkinje cell axons and presynaptic terminals in organotypic cerebellar cultures exposed to ionizing irradiation (1981)
    Springer
    Acta neuropathologica 54 (1981), S. 301-310 
    Details
    ISSN: 1432-0533
    Keywords: Dystrophic axons ; Ionizing irradiation ; Tissue culture ; Presynaptic terminals
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Ionizing irradiation induced marked morphological alterations in the Purkinje cell axons and axonic terminals in vitro. The myelinated segments demonstrated a very poor development of the myelin sheath. The axoplasm demonstrated various alterations of the mitochondria, and the smooth endoplasmic reticulum and the numerous elongated presynaptic terminals contained large aggregates of tubulovesicular structures and neurofilaments arranged in parallel and reticular array.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00697004
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Herpes simplex virus replication in pheochromocytoma cell line that responds to nerve growth factor (1978)
    Springer
    Acta neuropathologica 44 (1978), S. 9-14 
    Details
    ISSN: 1432-0533
    Keywords: Herpes simplex virus ; Replication in a pheochromocytoma cell line ; Nerve growth factor ; Morphological changes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Cultured cells of neural origin (PC-12, pheochromocytoma cell line) respond to nerve growth factor (NGF) by extending neurites. These cells whether treated with NGF or not can be infected with herpes simplex virus and produce progeny virus. Viral antigens are detected on the cell surface and fusion of cells to form polykaryocytes takes place. Nucleocapsids are found within the cell nucleus and enveloped virus in present both in the cytoplasm and extracellular space. Virus was not observed within the neurites but alterations in the neurite microtubular structure occurred after infection.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00691633
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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