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  • Bulk isolation  (1)
  • Dystrophic axons  (1)
  • Growth hormone  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Isolation, culture and cell-type identification of adult human pituitary cells (1985)
    Springer
    Acta neuropathologica 68 (1985), S. 205-208 
    Details
    ISSN: 1432-0533
    Keywords: Human pituitary gland ; Tissue culture ; Density gradients ; Immunocytochemistry ; Growth hormone ; Prolactin ; Adrenocorticotropin ; Follicle-stimulating hormone ; Luteinizing hormone ; Thyrotropin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Pituitary cells were isolated from adult human pituitary glands obtained at autopsy 4–12 h postmortem by enzyme treatment (collagenase and dispase) and by Percoll density gradients. Cells thus isolated were maintained in culture for more than 6 months. By immunoperoxidase staining methods using rabbit sera monospecific against various pituitary hormones, a large number of cultured cells reacted positively. The hormones identified in these cells were growth hormone, prolactin, adrenocorticotropin, follicle-stimulting hormone, luteinizing hormone and thyrotropin. Electron-microscopic examination of cultured cells revealed the presence of secretory granules in cytoplasm characteristic of in vivo human pituitary cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00690196
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Morphological alterations of Purkinje cell axons and presynaptic terminals in organotypic cerebellar cultures exposed to ionizing irradiation (1981)
    Springer
    Acta neuropathologica 54 (1981), S. 301-310 
    Details
    ISSN: 1432-0533
    Keywords: Dystrophic axons ; Ionizing irradiation ; Tissue culture ; Presynaptic terminals
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Ionizing irradiation induced marked morphological alterations in the Purkinje cell axons and axonic terminals in vitro. The myelinated segments demonstrated a very poor development of the myelin sheath. The axoplasm demonstrated various alterations of the mitochondria, and the smooth endoplasmic reticulum and the numerous elongated presynaptic terminals contained large aggregates of tubulovesicular structures and neurofilaments arranged in parallel and reticular array.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00697004
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Culture and cryopreservation of adult human oligodendrocytes and astrocytes (1984)
    Springer
    Acta neuropathologica 64 (1984), S. 172-175 
    Details
    ISSN: 1432-0533
    Keywords: Adult human ; Oligodendrocyte ; Astrocyte ; Tissue culture ; Cryopreservation ; Bulk isolation ; Immunofluorescence microscopy ; Cell type-specific markers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Human adult oligodendrocytes were bulkisolated from the brains obtained at autopsy by the Percoll density gradient method and cultured in vitro for 2–28 days. Cultured human oligodendrocytes were then scraped from the dishes or coverslips, frozen at −70°C in the presence of 10% dimethyl sulfoxide as a cryoprotectorant, and then stored in liquid nitrogen tank. After the fast thawing, 55%–70% of human oligodendrocytes plus astrocytes were found to survive in the culture. Identification of cell types was established by using antibodies directed against galactocerebroside (oligodendrocyte) and glial fibrillary acidic protein (astrocyte).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00695582
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    Paper (German National Licenses)
    Fulltext
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