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  • Bulked segregant analysis  (1)
  • Key words Rfp1 restorer gene  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Genetic mapping of nuclear fertility restorer genes for the ‘Polima’ cytoplasmic male sterility in canola (Brassica napus L.) using DNA markers (1997)
    Springer
    Theoretical and applied genetics 95 (1997), S. 321-328 
    Details
    ISSN: 1432-2242
    Keywords: Key words Rfp1 restorer gene ; Rfp2 restorer gene ; pol CMS ; RFLP mapping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Co-segregation of male fertility with DNA markers selected by targeted mapping approaches as being potentially linked to the Rfp1 restorer gene for the pol cytoplasmic male sterility (CMS) was analyzed using two canola (Brassica napus L.) backcross populations. Eleven DNA markers (10 RFLP markers and one RAPD marker) directly linked to the Rfp1 locus were identified. The linkage group containing the Rfp1 locus was found to correspond to B. napus linkage group 18 of Landry et al. (1991). A similar pattern of co-segregation between DNA markers and male fertility was observed in a backcross population segregating for the pol restorer gene Rfp2 from line ‘UM2383’; one RFLP marker, cRF1b, showed perfect linkage with both Rfp1 and Rfp2 and detected identical polymorphic fragments in both the Rfp1 and Rfp2 restorer lines. Our findings indicate that restoration of pol CMS is controlled by a single nuclear genetic locus on linkage group 18 and that Rfp1 and Rfp2 are likely allelic.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050566
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Targeted mapping approaches to identify DNA markers linked to the Rfp1 restorer gene for the ‘Polima’ CMS of canola (Brassica napus L.) (1998)
    Springer
    Theoretical and applied genetics 97 (1998), S. 431-438 
    Details
    ISSN: 1432-2242
    Keywords: Key words Targeted mapping ; RFLP ; RAPD ; Brassica napus ; Polima CMS ; Nearly isogenic line ; Bulked segregant analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  We have used two targeting approaches [pairs of nearly isogenic lines (NILs) and bulked segregant analysis] to identify DNA markers linked to the Rfp1 restorer gene for the pol CMS of canola (Brassica napus L.). We were able to target the Rfp1 locus as efficiently by comparing NILs as by bulked segregant analysis, and it was demonstrated in this instance that double-screening strategies could significantly improve the overall targeting efficiency. The chance occurrence of shared homozygosity at specific unlinked chromosomal regions in the bulks was found to limit the efficiency of bulked segregant analysis, while the efficiency of NIL comparison was limited by residual DNA from the donor cultivar at scattered sites throughout the genome of the NILs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050913
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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