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  • 1
    ISSN: 1423-0127
    Keywords: Morphine ; Butorphanol ; Dependence, physical ; Glutamate ; Locus coeruleus ; Microdialysis ; Protein kinases
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract To investigate the role of glutamate in the locus coeruleus (LC) during opioid withdrawal, rats were continuously infused with morphine (a μ-opioid receptor agonist, 26 nmol/µl/h) or butorphanol (a μ/δ/κ-mixed opioid receptor agonist, 26 nmol/µl/h) intracerebroventricularly (i.c.v.) via osmotic minipumps for 3 days. A direct LC injection of glutamate (1 or 10 nmol/5 µl) or naloxone (an opioid receptor antagonist, 24 nmol/5 µl) induced withdrawal signs in morphine- or butorphanol-dependent animals. However, these agents failed to precipitate any withdrawal signs in saline-treated control animals. On the other hand, the expression of withdrawal signs precipitated by the administration of glutamate or naloxone in opioid-dependent animals was completely blocked by concomitant infusion with 1 or 10 nmol/µl/h of an inhibitor of adenosine 3′,5′-cyclic monophosphate (cAMP)-dependent protein kinase and protein kinase C, H-7 [1-(5-isoquinolinesulfonyl)-2-methylpiperazine]. In animals that had been infused with opioids in the same manner, i.c.v. injection of naloxone (48 nmol/5 µl) precipitated withdrawal signs and increased extracellular fluid levels of glutamate in the LC of morphine- or butorphanol-dependent rats measured by in vivo microdialysis method. However, concomitant infusion with H-7 inhibited the increases of glutamate levels in the LC. These results strongly suggest that an expeditious release of glutamate in the LC region plays an important role in the expression of physical dependence on opioids. Furthermore, the action on glutamate release might be increased by the enhancement of cAMP-dependent protein kinase and/or protein kinase C activity.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Journal of paleolimnology 14 (1995), S. 337-354 
    ISSN: 1573-0417
    Keywords: pollen ; palaeoecology ; paleoclimate ; glacial ; interglacial ; DCA ; North America
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences
    Notes: Abstract Raymond Basin and Bald Knob Basin, Montgomery County, Illinois, formed as kettles during Illinoian time. Fossil pollen from these basins provides information on vegetation and climate during the last glacial-interglacial cycle. The pollen profile at Raymond Basin contains an expanded Sangamonian section and an early Wisconsinan section, but both are missing the late Wisconsinan. The ages for the following pollen zones are estimated by correlation with the deep-sea δ18O record. In the late Illinoian,Picea-Pinus pollen zone 1, dating from about 150–130 ka, represents an apparently closed boreal coniferous forest indicating a cold late-glacial climate. The Sangamonian includes three major pollen zones ranging from about 130–75 ka. The early Sangamonian is represented byQuercus-Ulmus-Carya-Fagus dominance in zone 2, indicating vegetation comparable to the modern deciduous forest and climate that was warm and moist. The middle Sangamonian in zone 3, which is characterized byAmbrosia-Poaceae-Cupressaceae-Quercus pollen, suggests a savanna vegetation and a warm, dry climate. The late Sangamonian is subdivided into aQuercus-Ulmus-Carya subzone (4a) that indicates a mesic forest and greater precipitation; aQuercus-Ambrosia subzone (4b) that suggests drier climate and savanna conditions; and aQuercus-Liquidambar-Carya subzone (4c), containing the ‘southeastern forest’ element,Liquidambar, suggesting the peak in warmth and moisture. The early Wisconsinan is represented by a transitionalPicea-Chenopodiineae zone (5). This unusual assemblage suggests a cool prairie-like vegetation, perhaps with scatteredPicea trees at the end of that depositional interval. The Middle Wisconsinan is marked by the return of high percentages ofPicea andPinus pollen in zone 6. The latest pollen zone (7) is dominated by Chenopodiineae pollen and is absent at Raymond Basin. It is most likely Holocene in age, and probably represents prairie conditions and warm, dry climate. Apparent surface-temperature and apparent effective-moisture curves were derived from the first detrended correspondence analysis (DCA) axis of the pollen data. The first axis correlates well with the normalized deep-sea δ18O curve of sea surface temperature, and the second is controlled mainly by precipitation. The interglacial vegetation differs from that predicted by models driven by orbitally-induced insolation curves.
    Type of Medium: Electronic Resource
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