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  • 1
    Digitale Medien
    Digitale Medien
    Springer
    Acta neuropathologica 16 (1970), S. 154-160 
    ISSN: 1432-0533
    Schlagwort(e): Hyperactivity ; Succinoxidase Activity ; Reticular Neurons ; β-β-Iminodipropionitrile
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Beschreibung / Inhaltsverzeichnis: Zusammenfassung Die Succinooxidaseaktivität einzelner Neurone aus der Formatio reticularis wurde mit der Cartesianischen Tauchertechnik gemessen. Die Durchschnittsaktivität der Nervenzellkörper des Nucleus gigantocellularis sowie des Nucleus pontis caudalis der Kontrollratten war 4,5 μl O2×10−4 pro Stunde bei 37°C. Nachdem die Ratten β-β-Iminodipropionitril bekommen hatten, stieg die Durchschnittsaktivität derselben Neurone bis auf 8.0 μl O2·10−4 an. Es wird angenommen, daß dieser Anstieg für die sich später entwickelnden Hyperaktivitätssymptome ätiologische Bedeutung besitzt. Auch wenn β-β-Iminodipropionitril zu reticulären Neuronen normaler Ratten in vitro gegeben wurde, stieg die Succinooxidaseaktivität bis auf 8,4 μl O2·10−4 pro Stunde an.
    Notizen: Summary The succinoxidase activity of single neurons from the reticular formation was measured with the Cartesian diver technique. The average activity of nerve cell bodies from the nucleus gigantocellularis or pontis caudalis from control rats was 4.5 μl O2×10−4 per hour at 37°C. After rats had received β-β-iminodipropionitrile, the succinoxidase activity of the same type neuron increased to an average of 8.0 μl O2×10−4. It was suggested that this increase was of etiological significance for the symptoms of hyperactivity which developed later. Also when β-β-iminodipropionitrile had been added to the reticular neurons from normal rats in vitro, the succinoxidase activity increased to 8.4 μl O2×10−4 per hour.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Springer
    Pflügers Archiv 422 (1993), S. 354-363 
    ISSN: 1432-2013
    Schlagwort(e): K+ channel inactivation ; N-type inactivation ; C-type inactivation ; Pore or P-type inactivation ; External TEA enhancement of current ; External K+ enhancement of current ; Conductance ; Pore mutations
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract An N-terminus peptide or a C-terminus mechanism involving a single residue in transmembrane segment 6 produces inactivation in voltage-dependent K+ channels. Here we show that a single position in the pore of K+ channels can produce inactivation having characteristics distinct from either N- or C-type inactivation. In a chimeric K+ channel (CHM), the point reversion CHM V 369I produced fast inactivation and CHM V 369S had the additional effect of halving K+ conductance consistent with a position in the pore. The result was not restricted to CHM; mutating position 369 in the naturally occurring channel Kv2.1 also produced fast inactivation. Like N- and C-types of inactivation, pore or P-type inactivation was characterized by short bursts terminated by rapid entry into the inactivated state. Unlike C-type inactivation, in which external tetraethylammonium (TEA) produced a simple blockade that slowed inactivation and reduced currents, in P-type inactivation external TEA increased currents. Unlike N-type inactivation, internal TEA produced a simple reduction in current and K+ occupancy of the pore had no effect. External TEA was not the only cation to increase current; external K+ enhanced channel availability and recovery from inactivation. Additional features of P-type inactivation were residue-specific effects on the extent of inactivation and removal of inactivation by a point reversion at position 374, which also regulates conductance. The demonstration of P-type inactivation indicates that pore residues in K+ channels may be part of the inactivation gating machinery.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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