ISSN:
1432-1335
Keywords:
CD28 lymphocyte stimulation
;
TGFβ
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary After simultaneous stimulation with anti-CD3 monoclonal antibody (mAb) at 10 ng/ml, anti-CD28 mAb at 125 ng/ml, and interleukin-2 (IL-2) at 20 U/ml, peripheral blood mononuclear cells (PBMC) were partially resistant to immunosuppression by transforming growth factor-β (TGFβ2). The doses of TGFβ2 that inhibit cytotoxicity of IL-2 stimulated cells by 60%–70% were much less effective when the same cells were stimulated with anti-CD3/anti-CD28/IL-2. This favorable stimulation generated a cell population characterized by high lytic activity, excellent expansion, and a greater resistance to immunosuppressive action of TGFβ2. The secretion of secondary cytokines important for LAK generation is considered a crucial event, at least partially responsible for the antagonization of TGFβ immunosuppression.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01229526
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