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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Research in experimental medicine 189 (1989), S. 163-171 
    ISSN: 1433-8580
    Keywords: G-CSF ; Monoclonal antibody ; ELISA ; CFU-GM
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary An IgG monoclonal antibody to recombinant human granulocyte colony-stimulating factor (G-CSF), designated HG1, was produced by fusion of immune mouse splenocytes with HAT-sensitive murine myeloma cells. This HG1 was capable of neutralizing the colony-stimulating activity of G-CSF in vitro, and it did not cross-react with human granulocyte/macrophage colony-stimulating factor (GM-CSF). An enzyme-linked immunosorbent assay (ELISA) for measurement of G-CSF was developed using HG1 and a polyclonal antibody against G-CSF raised in a rabbit. The data indicated that the ELISA was highly efficient and sensitive for the detection of as little as 50pg/ml of recombinant G-CSF. This assay system therefore warrants further attention.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Research in experimental medicine 188 (1988), S. 405-409 
    ISSN: 1433-8580
    Keywords: Silica particle ; CFU-C ; Endogenous CSF
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A technique employing silica particles was used to remove cells producing endogenous colony-stimulating factor (CSF) to allow measurement of the level of colony-forming units in culture (CFU-C) in human bone marrow cells. In comparison with the glass-adherence technique, this new approach resulted in a more complete degree of removal of CSF-producing cells and formation of more colonies. This method seems to be useful for performing an accurate assay of exogenous CSF.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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