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  • 1
    ISSN: 1432-203X
    Keywords: Key words PCR ; RAPD analysis ; Corylus avellana ; Cultivars
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The random amplified polymorphic DNA (RAPD) technique offers a useful tool to detect DNA polymorphisms. It can also be used to distinguish different clones and cultivars. We have developed a comprehensive RAPD-based procedure for the routine molecular typing of various plants. Here we report the application of this technique for the correct identification of six hazelnut cultivars (Corylus avellana) widespread in the Campania region (south Italy). The analysed hazelnut cultivars were successfully distinguished by their RAPD fingerprints using the DNA primers U2, U3, U4, U11 and U14. However, in each cultivar we observed very low genetic heterogeneity among the clonal variants. Since this technique is among the simplest and easiest methods used to fingerprint DNA, it could be easily transferred to less sophisticated laboratory infrastructures (e.g. outstations of crop regulatory agencies).
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Analog integrated circuits and signal processing 8 (1995), S. 145-155 
    ISSN: 1573-1979
    Keywords: digital-to-analog conversion ; CMOS steering-current techniques ; multiplying functions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Electrical Engineering, Measurement and Control Technology
    Notes: Abstract This paper discusses the design and integrated circuit implementation of two alternative solutions for realizing an 8-bit high-speed steering-current multiplying digital-to-analog converter based on a current-source-array architecture. In the first solution, designated as voltage-controlled-current-source operation, a reference voltage is used to drive directly the gate-to-source voltage of the current-sources while in the second solution, designated as geometry-programmable-current-source operation, the current-sources are generated using a digitally programmable transistor-array. The characteristics of both solutions are discussed from the viewpoints of the accuracy and distortion introduced by the multiplying function. For demonstration purposes a prototype chip suitable for both operating modes has been fabricated in a single-poly 1.2µm CMOS digital technology. At 5 V supply and 13.3 mA full-scale output current, the chip dissipates about 90 mW for a sampling frequency of 50 MHz. The die area is approximately 1.75 mm2.
    Type of Medium: Electronic Resource
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