ISSN:
1573-5168
Keywords:
COMT
;
gonadotropic cells
;
dopamine
;
catecholestrogens
;
Clarias gariepinus
;
teleosts
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Isolated gonadotrops of the African catfish,Clarias gariepinus, were incubated with dopamine (DA) and/or catecholestrone and the activity of the enzyme catechol-O-methyltransferase (COMT) was determined by measuring the methylated products. From the apparent Km values for DA and catecholestrone of 0.4–1.3 μM and 17.9–25.2 μM respectively, it was concluded that catecholestrone is a better substrate for the enzyme COMT, compared to DA. Moreover, the methylation of DA is inhibited by comparatively low concentrations of catecholestrone.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01875023
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