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Differential Responsiveness to Contractile Agents of Isolated Smooth Muscle Cells from Human Colons as a Function of Age and Inflammation (1997)

Boyer, Jean-Christophe ; Guitton, Claire ; Pignodel, Christine ; [et al.]

Springer

Digestive diseases and sciences 42 (1997), S. 2190-2196

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ISSN: 1573-2568

Keywords: SMOOTH MUSCLE CELLS ; COLON ; CONTRACTION ; CCK ; MUSCARINIC ; AGONISTS

Source: Springer Online Journal Archives 1860-2000

Topics: Medicine

Notes: Abstract To study the involvement of age and inflammationin motor colonic activity in man, contractile responsesto CCK, carbachol, and KCl of isolated colonic smoothmuscle cells (SMC) from normal and inflamed human colons were evaluated; the incidence ofsex and smoking on contraction was also analyzed.Contractile responses to the three agonists weresignificantly lower in tissues with a low degree ofinflammation than in tissues with high level of inflammationor normal tissues. This reduction in cell responsivenessappears to be nonspecific and nonreceptor mediated. Apositive correlation of the contractile responses to the three stimulants with the age ofpatients was observed. In contrast, no association wasfound between sex, smoking, and cell contraction. Inconclusion, contractions of SMC due to CCK, carbachol, and KCl were significantly modified duringlife; inflammation of the colon led to a loss of SMCresponsiveness.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1023/A:1018894029140

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Lipases operative at the fat cell surface: Attempt at an integrated approach (1987)

Verine, Alain ; Boyer, Jean

New York, NY [u.a.] : Wiley-Blackwell

Cell Biochemistry and Function 5 (1987), S. 175-181

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ISSN: 0263-6484

Keywords: Lipoprotein lipase ; diester lipase ; monoeser lipase ; oleic acid uptake ; cell lipids ; apolipoproteins ; female rats ; Chemistry ; Biochemistry and Biotechnology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Medicine

Notes: Extracellular acylglycerols are hydrolysed by lipases active at the surface of intact fat cells isolated from rat or human adipose tissue. During short-term incubation, rat fat cells hydrolyse di-[3H]oleyl-[14C]glycerol at a rate of 70 ± 7·7 mU/106 cells (means ± S.E.) versus 440 ± 62 mU/106 cells for the hydrolysis of mono-[3H]oleylglycerol; these relatively high lipolytic potencies may serve, among other functions, to counteract the cytolytic effect of both esters. Reaction rates with both substrates are unchanged by addition of various apolipoproteins C and by the nutritional state of the animals. Fat cells incorporate 15-20 per cent of the total [3H]-oleic chains liberated by hydrolysis, with no correlation between uptake and hydrolysis rates. [3H]-oleic chains in cell lipids are found mainly as diacylglycerol (15 per cent) and triacylglycerol (80 per cent). Both lipolytic processes differ from the hydrolysis of trioleyglycerol by cell-bound lipoprotein lipase, which occurs at lower rates (6·5 ± 0·6 mU/106 cells) and depends on apolipoprotein C-II and nutritional state of the animals. The results support the accepted view that lipoprotein lipase and monoacylglycerol lipase are distinct enzymes. Differences between lipoprotein lipase and diacylglycerol lipase activities raise the possibility of different catalytic entities. In conclusion, isolated fat cells in suspension hydrolyse and incorporate lipids. This model should approximate physiological conditions more closely than the use of lipases in the free state.

Additional Material: 3 Ill.