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  • Ca2+/calmodulin-dependent protein kinase II  (1)
  • Column liquid chromatography  (1)
  • Enzymeimmobilization  (1)
  • Geographical distribution  (1)
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  • 1
    Digitale Medien
    Digitale Medien
    Ca2+/calmodulin-dependent protein kinase II immunoreactivity in Lewy bodies (1991)
    Springer
    Acta neuropathologica 82 (1991), S. 159-163 
    Details
    ISSN: 1432-0533
    Schlagwort(e): Lewy body ; Lewy body-like hyaline inclusion ; Ca2+/calmodulin-dependent protein kinase II ; Parkinson's disease ; Amyotrophic lateral sclerosis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Ca2+/calmodulin-dependent protein kinase II (CaM kinase II) is one of the predominant protein kinases in the brain. We found that CaM kinase II immunoreactivity was concentrated in the peripheral halos of Lewy bodies (LBs) in Parkinson's disease and Lewy body-like hyaline inclusions (LBHIs) in amyotrophic lateral sclerosis. An immunoelectron microscopic examination of LBs revealed that the filaments at the periphery of LBs were decorated with immunopositive deposits. Since CaM kinase II has a broad substrate specificity and can phosphorylate neurofilaments and other cytoskeletal proteins, it may play some role in the formation of LBs and LBHIs through the aberrant phosphorylation of the cytoskeletal elements in these inclusions.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00294440
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Ribosomal DNA variation in foxtail millet, Setaria italica (L.) P. Beauv., and a survey of variation from Europe and Asia (1997)
    Springer
    Theoretical and applied genetics 95 (1997), S. 751-756 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Foxtail millet ; rDNA ; RFLP ; Restriction mapping ; Geographical distribution
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  Restriction fragment length polymorphism (RFLP) and the structure of ribosomal RNA genes (rDNA) were investigated in 117 landraces of foxtail millet, Setaria italica (L.) P. Beauv. Five RFLP phenotypes were found when the genomic DNA was digested with BamHI; these were named types I–V. Of these types I, II and III were the most frequent. Type I was mainly distributed in the temperature zone, type II in the Taiwan-Philippines Islands and type III in South Asia. Restriction mapping of the cloned rDNA and comparison with RFLP phenotypes showed that the different types originated from a polymorphism in the length within the intergenic spacer (IGS) and BamHI site changes within the IGS.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220050621
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Covalent immobilization of trypsin onto poly(2-hydroxyethyl methacrylate)/polystyrene composite microspheres by cyanogen bromide method and its enzymatic activity (1987)
    Springer
    Colloid & polymer science 265 (1987), S. 957-964 
    Details
    ISSN: 1435-1536
    Schlagwort(e): Enzymeimmobilization ; trypsin ; cyanogen bromide ; emulsion ; microsphere
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie , Maschinenbau
    Notizen: Abstract Covalent immobilization of trypsin onto poly(2-hydroxyethyl methacrylate)/ polystyrene composite microspheres, produced by emulsifier-free seeded emulsion polymerization technique, was carried out using the cyanogen bromide method under various conditions. The highest enzymatic activities of the trypsin immobilized in this study against N-α-benzoyl-L-arginine ethyl ester and N-α-benzoyl-L-arginine-p-nitroanilide, as low-molecular substrates and casein as a high-molecular substrate, were high, as corresponding to 80%, 85% and 50% of those of free trypsin, respectively.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01412397
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 4
    Digitale Medien
    Digitale Medien
    Determination of reduced-form glutathione and total glutathione in blood and plasma by high performance liquid chromatography with on-column fluorescence derivatization (1998)
    Springer
    Chromatographia 48 (1998), S. 690-694 
    Details
    ISSN: 1612-1112
    Schlagwort(e): Column liquid chromatography ; On-column fluorescence derivatization ; Blood and plasma ; Glutathione ; ortho-Phthalaldehyde
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Summary An on-column fluorometric derivatization method has been developed for the determination of reduced-form glutathione and total glutathione in blood and plasma by high-performance liquid chromatography. Blood hemolysate or diluted plasma was deproteinized with percholoric acid. For total glutathione determination, any oxidized forms of glutathione in samples were converted to reduced-form glutathione with dithiothreitol prior to protein precipitation. Deproteinized samples were injected without pretreatment. Glutathione was fluorescence-derivatized and separated on a C18-bonded vinyl alcohol polymer column withortho-phthalaldehyde in sodium borate buffer-acetonitrile, as mobile phase; theortho-phthalaldehyde-glutathione, fluorescent isoindole adduct is measured by its fluorescence response. Due to the high sensitivity, selectivity reproducibility and the simple HPLC system, the method is particularly suitable for routine assay of numerous or small amounts of samples.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02467600
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