ISSN:
1432-2013
Keywords:
Aluminum
;
Fluorine
;
Vanadate
;
Colon
;
Chloride secretion
;
Ca2+
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Stripped rabbit descending colon mucosae were studied in vitro in modified Ussing chambers to determine the effects of AlF 4 − and vanadate on Cl− transport. Serosal additions of AlF 4 − and vanadate increase short-circuit current (I SC) and tissue conductance, while luminal addition of the agents is ineffective. Addition of aluminium potentiates the effect of NaF on I SC. AlF 4 − and vanadate increase serosal-to-mucosal flux of 36Cl without affecting mucosal-to-serosal flux. The effects of these agents on I SC are markedly inhibited by serosal addition of bumetanide and depend on the presence of Na+ in the serosal bathing solution. The effects of AlF 4 − and vanadate on I SC are dependent on the presence of Ca2+ in the bathing solution, and are completely inhibited by indomethacin, but the effect of forskolin is not affected by the removal of Ca2+ from the bathing solution and the addition of indomethacin. AlF 4 − and vanadate significantly increase the level of inositol phosphate metabolites. The results indicate that AlF 4 − and vanadate increase Cl− secretion in the rabbit colon via an increase in prostaglandin synthesis which is mediated by the increase of intracellular Ca2+ concentrations.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00374627
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