ISSN:
1432-2013
Schlagwort(e):
Ca2+-activated K+ channels
;
Maxi-K+ channel
;
Tetraethylammonium
;
Salivary gland
;
Fluid secretion
;
K+ efflux
;
Epithelial transport
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Medizin
Notizen:
Abstract The involvement of large-conductance, voltage- and Ca2+-activated K+ channels (maxi-K+ channels) in basolateral Ca2+-dependent K+-efflux pathways and fluid secretion by the rat submandibular gland was investigated. Basolateral K+ efflux was monitored by measuring the change in K+ concentration in the perfusate collected from the vein of the isolated, perfused rat submandibular gland every 30 s. Under conditions in which the Na+/K+-ATPase and Na+-K+-2Cl− cotransporter were inhibited by ouabain (1 mmol/l) and bumeta-nide (50 μmol/l) respectively, continuous stimulation with acetylcholine (ACh) (1 μmol/l) caused a transient large net K+ efflux, followed by a smaller K+ efflux, which gradually returned to the basal level within 10 min. These two components of the K+ efflux appear to be dependent on an increase in cytosolic Ca2+ concentration. The initial transient K+ efflux was not affected by charybdotoxin (100 nmol/l) or tetraethylammonium (TEA) (5 mmol/l) but the smaller second component was strongly and reversibly inhibited by charybdotoxin (100 nmol/l) and TEA (0.1 and 5 mmol/l). The initial K+ efflux transient induced by ACh was inhibited by quinine (0.1–3 mmol/l), quinidine (1–3 mmol/l) and Ba2+ (5 mmol/l), but not by verapamil (0.1 mmol/l), lidocaine (1 mmol/l), 4-aminopyridine (1 mmol/l) or apamin (1 μmol/l). Ca2+-dependent transient large K+ effluxes induced by substance P (0.01 μmol/l) and A23187 (3 μmol/l) were not inhibited by TEA (5 mmol/l or 10 mmol/l). A23187 (3 μmol/l) evoked a biphasic fluid-secretory response, which was not inhibited by TEA (5 mmol/l). Patch-clamp studies confirmed that the whole-cell outward K+ current attributable to maxi-K+ channels obtained from rat submandibular endpiece cells was strongly inhibited by the addition of TEA (1–10 mmol/l) to the bath. It is concluded that maxi-K+ channels are not responsible for the major part of the Ca2+-dependent basolateral K+ efflux and fluid secretion by the rat submandibular gland.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF00374573
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