ISSN:
0749-1581
Keywords:
1H NMR
;
15N NMR
;
Calbindin D9k
;
Cadmium
;
Calcium
;
EF-hand calcium-binding proteins
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
The amide 15N and 1H chemical shifts of (Cd2+)2-calbindin D9k are presented. A detailed comparison is made with those of (Ca2+)2-calbindin D9k, showing that the (Cd2+)2 and (Ca2+)2 states of the protein are very similar, with differences predominantly located in ion-binding loop I. The present studies were carried out on the P43G mutant calbindin D9k, and corroborate previous conclusions based on a comparative analysis of the 1H chemical shifts of the (Cd2+)2 and (Ca2+)2 states of wild-type calbindin D9k. A value for the rate constant for the dissociation of cadmium from ion-binding loop I in calbindin D9k of koff ≍ 0.6 × 103 ± 0.2 × 103 was estimated by analyzing line broadening in protein samples containing equal amounts of (Cd2+)1- and (Cd2+)2-calbindin D9k.
Additional Material:
3 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/mrc.1260311324
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