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  • 1
    Electronic Resource
    Electronic Resource
    Cathepsin D: Ultra-immunohistochemical localization in dentinogenesis (1979)
    Springer
    Calcified tissue international 29 (1979), S. 251-256 
    Details
    ISSN: 1432-0827
    Keywords: Cathepsin ; Calcification ; Dentinogenesis ; Proteoglycans
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary Cathepsin D was purified from rat liver using a new affinity chromatographic method, based on the coupling to the specific inhibitor pepstatin. This preparation was used for the production of specific antibodies from rabbit. The purified IgG fraction was conjugated to horseradish peroxidase in a two-step coupling procedure and used for electron microscopic immunohistochemistry of the odontoblast-predentine region of the rat incisor. Precipitates, indicating the presence of cathepsin D, were seen in the odontoblast, odontoblast process, and in the extracellular unmineralized matrix, the predentine. The observations are discussed in relation to proteoglycan degradation at the mineralization front simultaneous with crystal formation, and in relation to the function of lysosomal enzymes in the turnover of connective tissue.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02408088
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Scanning electron microscopic studies of the rat incisor odontoblastema (1975)
    Springer
    Cell & tissue research 159 (1975), S. 233-243 
    Details
    ISSN: 1432-0878
    Keywords: Odontoblasts ; Predentine ; Dentine ; Calcification ; Scanning electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A scanning electron microscopic technique was used to investigate the surface structure of dentinogenically active odontoblasts. Thin pieces of rat incisors were fixed, rapidly frozen, freezedried at -70° C and fractured to expose new surfaces prior to examination in the SEM. Differences in the appearance of odontoblastic cell surfaces were seen, with the most extensive ridge formations at the distal part of the sides of the odontoblasts. The predentine area displayed a spongy structure which contrasted to the compact appearance of dentine. Results are discussed in relation to previous studies at the light microscopic and transmission electron microscopic levels.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00219159
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Ultrastructural localisation of proteoglycans in the odontoblast-predentin region of rat incisor (1976)
    Springer
    Cell & tissue research 168 (1976), S. 277-287 
    Details
    ISSN: 1432-0878
    Keywords: Proteoglycans ; Odontoblasts, predentin, dentin ; Calcification ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The localization of proteoglycans in the predentin of the rat incisor was investigated by ultrastructural histochemistry. Ruthenium red stained the cell coat of the odontoblasts as well as intracellular vesicles. There was also a staining of the extracellular matrix, but not of collagen fibers in the predentin. Treatment with the enzyme hyaluronidase prior to staining with ruthenium red abolished the staining of the vesicles and the extracellular matrix but not that of the cell coat. Bismuth nitrate and phosphotungstic acid gave similar staining of odontoblast vesicles and extracellular matrix. It is likely that the stained structures contain proteoglycans. The importance of these proteoglycans and their ultrastructural localization are discussed in relation to intracellular transport and the calcification process.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00215306
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    Paper (German National Licenses)
    Fulltext
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