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  • Calcium regulation Frog Sartorius muscle Skeletal muscle Synchrotron radiation Tropomyosin Twitch Two-dimensional X-ray diffraction  (1)
  • Expression in germ cells and epithelial cells  (1)
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  • 1
    Electronic Resource
    Electronic Resource
    Expression and evolutionary conservation of nanos-related genes in Hydra (2000)
    Springer
    Development genes and evolution 210 (2000), S. 591-602 
    Details
    ISSN: 1432-041X
    Keywords: Keywords Hydra ; nanos-related genes ; Expression in germ cells and epithelial cells ; Evolutionary conservation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The Drosophila gene nanos encodes two particular zinc finger motifs which are also found in germline-associated factors from nematodes to vertebrates. We cloned two nanos (nos)-related genes, Cnnos1 and Cnnos2 from Hydra magnipapillata. Using whole-mount in situ hybridization, the expression of Cnnos1 and Cnnos2 was examined. Cnnos1 was specifically expressed in multipotent stem cells and germline cells, but not in somatic cells. Cnnos2 was weakly expressed in germline cells and more specifically in the endoderm of the hypostome where it appears to be involved in head morphogenesis. In addition to structural conservation in the zinc finger domain of nanos-related genes, functional conservation of Cnnos1 was also demonstrated by the finding that a Cnnos1 transgene can partially rescue the nos RC phenotype that is defective in the egg production of Drosophila. Thus, the function of nanos-related genes in the germline appears to be well conserved from primitive to highly evolved metazoans.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004270000105
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Time-resolved two-dimensional X-ray diffraction study of the effect of shortening on activation of contracting skeletal muscle (2000)
    Springer
    Pflügers Archiv 439 (2000), S. 646-649 
    Details
    ISSN: 1432-2013
    Keywords: Calcium regulation Frog Sartorius muscle Skeletal muscle Synchrotron radiation Tropomyosin Twitch Two-dimensional X-ray diffraction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. In twitch contractions of frog skeletal muscle, the isometric tension peaks when intracellular calcium has fallen to near-resting levels. To understand the mechanism of this delayed tension maintenance in the context of calcium regulation, the time course of the tropomyosin movement on actin was monitored by recording the intensity of the 2nd actin layer lines in a time-resolved two-dimensional X-ray diffraction study. The intensity rose ahead of tension, reflecting the tropomyosin movement from its "off" to "on" positions, but it fell with a time course similar to that of tension. Muscle shortening applied at the tension peak was followed by a poor recovery of tension, and accelerated the fall of the reflection intensity. The results suggest that the force-generating myosin heads retain the tropomyosin in its "on" position after the fall of intracellular calcium, and their shortening-induced detachment makes the tropomyosin return to its "off" position, thereby preventing myosin reattachment to actin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004249900224
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    Paper (German National Licenses)
    Fulltext
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