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  • 1
    ISSN: 1573-4927
    Keywords: Calliphora ; calliphorin ; storage protein ; hemolymph ; fat body ; mRNA ; in vitro translation ; in situ hybridization ; structural gene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract A major poly(A)-containing RNA fraction of the approximate size expected of a monocistronic mRNA for the storage protein calliphorin has been isolated from the larval fat bodies of Calliphora vicina during early instar 3. This 20 S RNA fraction programs the synthesis by cell-free wheat embryo extracts of polypeptides of 86,000 daltons identified by tryptic peptide fingerprinting as precursors of the authentic calliphorin subunits of 83,000 daltons. Complementary DNA synthesized by AMV reverse transcriptase using the same 20 S RNA as template hybridized in situ to a single segment of one or two bands in the salivary polytene chromosomes of C. vicina.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    European journal of applied physiology 36 (1977), S. 207-213 
    ISSN: 1439-6327
    Keywords: Anaerobic training ; Human skeletal muscle ; Phosphagens ; LDH ; Anaerobic capacity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The effects of a 6 week program of intense, intermittent hill running was investigated in 5 endurance-trained men (34–37 years). Venous lactate responses averaged 10.7 mM×1−1 during training. Biopsies were obtained from the vastus lateralis before and after training for determinations of histochemical classification, phosphagen concentrations (ATP and CP) and activities of total LDH and selected LDH isozymes. Indices of aerobic and anaerobic capacity were also measured. Significant improvements (P〈0.01) were noted in training distance and loads employed in leg presses, also part of the training: the treadmill test for anaerobic capacity improved by 16.7% and the terminal blood lactate level increased 14% (P〈0.05). No changes occurred in $$\dot V$$ O2 max, body fat per cent or anaerobic power. Muscle ATP concentration increased by 14.8% (P〈0.01) whereas the tissue level of CP remained unchanged. Total LDH activity and the LDH isozymes (LDH-1, LDH-5 and LDH-2, 3, 4) were not altered by training. No changes in fibre distribution were observed. That adaptations to the anaerobic training did occur is evident, but the cellular locus remains to be elucidated further.
    Type of Medium: Electronic Resource
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