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  • 1
    Electronic Resource
    Electronic Resource
    Cloning and expression analysis of the plastidic fructose-1,6-bisphosphatase coding sequence from potato: circumstantial evidence for the import of hexoses into chloroplasts (1992)
    Springer
    Planta 188 (1992), S. 7-12 
    Details
    ISSN: 1432-2048
    Keywords: Calvin cycle ; Chloroplast ; Fructose-1,6-bisphosphatase ; Solanum (chloroplast) ; Sucrose induction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A copy DNA encoding the plastid-located isoform of the fructose-1,6-bisphosphatase (cp-FBPase) has been cloned from potato (Solanum tuberosum L.). Sequence analysis reveals a high degree of homology to cp-FBPases from wheat, spinach, andArabidopsis. Analysis of RNA blots shows that the expression of the cp-FBPase is limited to green tissue such as leaf and stem, and is absent from photosynthetically inactive tissue such as roots, tubers and stolons. This provides additional evidence that hexoses or hexose phosphates are imported into amyloplasts of heterotrophic tissues. Incubation of detached leaves of potato in darkness in a sucrosecontaining medium leads to massive accumulation of both starch and transcripts encoding starch biosynthetic enzymes. However, no transcripts encoding the cp-FBPase are detectable under these conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01160706
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Cloning and expression analysis of the plastidic fructose-1,6-bisphosphatase coding sequence from potato: circumstantial evidence for the import of hexoses into chloroplasts (1992)
    Springer
    Planta 188 (1992), S. 7-12 
    Details
    ISSN: 1432-2048
    Keywords: Calvin cycle ; Chloroplast ; Fructose-1,6-bisphosphatase ; Solanum (chloroplast) ; Sucrose induction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A copy DNA encoding the plastid-located isoform of the fructose-1,6-bisphosphatase (cp-FBPase) has been cloned from potato (Solanum tuberosum L.). Sequence analysis reveals a high degree of homology to cp-FBPases from wheat, spinach, and Arabidopsis. Analysis of RNA blots shows that the expression of the cp-FBPase is limited to green tissue such as leaf and stem, and is absent from photosynthetically inactive tissue such as roots, tubers and stolons. This provides additional evidence that hexoses or hexose phosphates are imported into amyloplasts of heterotrophic tissues. Incubation of detached leaves of potato in darkness in a sucrosecontaining medium leads to massive accumulation of both starch and transcripts encoding starch biosynthetic enzymes. However, no transcripts encoding the cp-FBPase are detectable under these conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00198933
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Reduced sedoheptulose-1,7-bisphosphatase levels in transgenic tobacco lead to decreased photosynthetic capacity and altered carbohydrate accumulation (1997)
    Springer
    Planta 204 (1997), S. 27-36 
    Details
    ISSN: 1432-2048
    Keywords: Key words: Antisense DNA ; Calvin cycle ; Carbon assimilation ; Nicotiana (transgenic) ; Sedoheptulose-1 ; 7-bisphosphatase ; Transgenic plant (tobacco)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. Transgenic tobacco (Nicotiana tabacum L. cv. Samsun) plants with reduced levels of the Calvin cycle enzyme sedoheptulose-1,7-bisphosphatase (SBPase; EC 3.1.3.37) were produced using an antisense construct in which the expression of a tobacco SBPase cDNA clone was driven by the cauliflower mosaic virus (CaMV) promoter. The reduction in SBPase protein levels observed in the primary transformants correlated with the presence of the antisense construct and lower levels of the endogenous SBPase mRNA. No changes in the amounts of other Calvin cycle enzymes were detected using Western blot analysis. The SBPase antisense plants with less than 20% of wild-type SBPase activity were observed to display a range of phenotypes, including chlorosis and reduced growth rates. Measurements of photosynthesis, using both light-dosage response and CO2 response curves, of T1 plants revealed a reduction in carbon assimilation rates, which was apparent in plants retaining 57% of wild-type SBPase activity. Reductions were also observed in the quantum efficiency of photosystem II. This decrease in photosynthetic capacity was reflected in a reduction in the carbohydrate content of leaves. Analysis of carbohydrate status in fully expanded source leaves showed a shift in carbon allocation away from starch, whilst sucrose levels were maintained in all but the most severely affected plants. Plants with less than 15% of wild-type SBPase activity were found to contain less than 5% of wild-type starch levels. The results of this preliminary analysis indicate that SBPase activity may limit the rate of carbon assimilation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004250050226
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    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Molecular biology of the C3 photosynthetic carbon reduction cycle (1991)
    Springer
    Photosynthesis research 27 (1991), S. 1-14 
    Details
    ISSN: 1573-5079
    Keywords: Calvin cycle ; chloroplast metabolism ; plant nuclear genes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In recent years the enzymes of the C3 photosynthetic carbon reduction (PCR) cycle have been studied using the techniques of molecular biology. In this review we discuss the primary protein sequences and structural predictions that have been made for a number of these enzymes, which, with the input of crystallographic analysis, gives the opportunity to understand the mechanisms of enzyme activity. The genome organisation and gene structure of the PCR enzymes is another area which has recently expanded, and we discuss the regulation of the genes encoding these enzymes and the complex interaction of various factors which influence their expression.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00029971
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    Paper (German National Licenses)
    Fulltext
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