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Protein-phosphatase inhibitors block root hair growth and alter cortical cell shape ofArabidopsis roots (1994)

Smith, R. D. ; Wilson, J. E. ; Walker, J. C. ; [et al.]

Springer

Planta 194 (1994), S. 516-524

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ISSN: 1432-2048

Keywords: Arabidopsis ; Calyculin-A ; Okadaic acid ; Protein phosphatase ; Root development

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract Emerging evidence suggests that protein phosphatases play an important role in the growth and development of higher plants. We report here on the effects of okadaic acid and calyculin-A, two specific and potent inhibitors of the type-1 and type-2A families of serine/ threonine protein phosphatases, on the growth and development ofArabidopsis thaliana L. roots. Application of these drugs in nanomolar ranges arrested root hair growth, severely affected the shape of cells within the zone of elongation and inhibited root growth rates. Root hair elongation was inhibited by concentrations of okadaic acid and calyculin-A as low as 3 nM. The pleiotropic effects of okadaic acid and calyculin-A point to multiple functions for type-1 and -2A protein phosphatases in controlling root growth and development.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00714465

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Rapid analysis using capillary supercritical fluid chromatography (1986)

Wright, B. W. ; Smith, R. D.

Weinheim : Wiley-Blackwell

Journal of High Resolution Chromatography 9 (1986), S. 73-77

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ISSN: 0935-6304

Keywords: Capillary supercritical fluid chromatography ; Fast analysis ; Nonvolatile analytes ; Chemistry ; Analytical Chemistry and Spectroscopy

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology

Notes: Capillary supercritical fluid chromatography (SFC) is proving to be a viable and useful separation method for thermally labile and nonvolatile materials. As with other capillary chromatographic techniques, very fast separations can be accomplished by sacrificing total efficiency and optimizing the conditions for rapid analysis. This is achieved using short, small-bore capillary columns, increased mobile phase linear velocities and very fast pressure programming rates. These principles are demonstrated for the rapid separation of selected component systems.

Additional Material: 4 Ill.