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  • Candida amapae  (1)
  • Collagen  (1)
  • Drosophila serido  (1)
  • 1
    ISSN: 1572-9699
    Keywords: Yeast communities ; Drosophila serido ; cactus ; feeding behavior
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The feeding behavior ofDrosophila serido on the yeast communities of necrotic stem tissue ofPilosocereus arrabidae were studied in a sand dune ecosystem of Rio de Janeiro, Brazil. The prevalence of cactophilic yeasts includingPichia barkeri, Candida sonorensis andGeotrichum sp. in the crops and external surfaces ofD. serido reflected its association with the cactus habitat. The effective number of yeasts vectored on the surface of flies was higher than that in the crops. Also overlap between the yeasts from stems and from crops was partial suggesting selective feeding by the flies in the substrates visited. The females had a higher effective number of yeast species and a lower similarity than males with the yeast community ofP. arrabidae. This was probably related to the search for oviposition sites by females. The presence ofPichia thermotolerans-like andPichia amethionina varpachycereana in the flies, but not inP. arrabidae stems, indicated thatD. serido was not limited to this cactus species. The larvae and adults lived in different patches with the adults feeding in patches with higher yeast species richness. The larvae had a narrower feeding niche and higher overlap withP. arrabidae, and preferredP. barkeri andPichia cactophila as food. Adult flies fed on patches with the most frequent yeasts except forP. cactophila. Pichia caribaea was found in higher frequency in the adult crops than in the stems. Our data suggested that there was food selection and diet partitioning between adult and larval stages ofD. serido.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-069X
    Keywords: Key words Retinoic acid ; Collagen ; Collagenase ; Stromelysin ; Photoaging
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Chronically sun-damaged human skin has a wrinkled, aged appearance as a result of alterations in the dermal extracellular matrix. Secondary effectors such as cytokines and integrins may mediate the effects of UV radiation on the skin by regulating the synthesis of metalloproteinases and structural proteins including collagen. The aim of this study was to semiquantify the steady-state mRNA levels of interleukin-1α, tumor necrosis factor α, transforming growth factor β, collagenase, stromelysin, collagen, and integrins (α 1 and α 2 ) in the skin of hairless mice that were either treated with UV or concurrently treated with UV and topical tretinoin for 5 weeks. Total RNA was extracted from the skin of the mice, reverse transcribed to cDNA, and amplified by the polymerase chain reaction in the presence of 32 P-dCTP using gene-specific primers. Results were normalized relative to glyceraldehyde-3-phosphate dehydrogenase levels. Steady-state mRNA levels of the cytokines and integrins were increased by UV radiation. Concurrent UV and topical tretinoin treatment superinduced the expression of interleukin-1, increased α 1 and decreased α 2 integrin expression. Immunofluorescence analysis showed increased dermal localization of β 1 integrin in UV and tretinoin treated skin. These results suggest that cytokines and integrins may be involved in the mechanism of photodamage.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Journal of industrial microbiology and biotechnology 14 (1995), S. 531-535 
    ISSN: 1476-5535
    Keywords: Methionine auxotrophy ; Amazonian fruit ; Candida amapae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary A new species of oligotrophie, methionine-or cysteine-requiring yeast was isolated from ripening fruits of the AmazonianParahancornia amapa tree. This species resemblesCandida sorboxylosa and species ofIssatchenkia but is nonfermentative and forms abundant pseudomycelia. The new species was isolated only fromP. amapa fruits, and not from other fruits or the drosophilid vectors of the yeast community of the deteriorating fruit. The taxon is regarded as indigenous to the amapa fruit or inoculated by vectors other thanDrosophila before ripening. A description of the new speciesCandida amapae is given.
    Type of Medium: Electronic Resource
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