Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Recent developments in capillary isoelectric focusing with whole-column imaging detection (1998)
    Weinheim : Wiley-Blackwell
    Electrophoresis 19 (1998), S. 2290-2295 
    Details
    ISSN: 0173-0835
    Keywords: Capillary isoelectric focusing ; Imaging detection ; On-line desalting ; Tapered capillary ; Protein analysis ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Capillary isoelectric focusing (CIEF) is a high resolution technique for protein separation. The on-column single point detector requires a mobilization step which lengthens the analysis time and causes an uneven resolution along the separation column. The real time and whole column imaging detection has been developed for performing CIEF without mobilization. Three types of imaging detection systems have been developed: optical absorption, refractive index gradient, and laser induced fluorescence. This technique provides a fast analysis speed (about 6 min) and a good resolution of 0.03 pH unit level. Using the absorption imaging detector, ampholyte-free IEF in tapered capillary is being demonstrated, which eliminates the interference of the expensive carrier ampholytes for protein detection in UV region. Recent advancements in this imaged CIEF technique as well as its applications are reviewed.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.1150191307
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Application of capillary isoelectric focusing with absorption imaging detection to the quantitative determination of human hemoglobin variants (1995)
    Weinheim : Wiley-Blackwell
    Electrophoresis 16 (1995), S. 670-673 
    Details
    ISSN: 0173-0835
    Keywords: Capillary electrophoresis ; Imaging detection ; Isoelectric focusing ; Hemoglobin ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: The capillary isoelectric focusing (CIEF)-absorption imaging detector is a multicapillary separation instrument. Several protein samples can be separated and detected in a single run. The analysis time for one run is only 3 min. Because an imaging detector is used, the mobilization process of conventional CIEF is not necessary, eliminating such drawbacks as long analysis time and poor reproducibility in mobilization speed. Human hemoglobin variants were quantitatively determined using the instrument. The peak areas of the analytes were proportional to their concentrations in the concentration range of 0-200 μg/mL. Hemoglobin variant A2, which only comprises 2% of the whole hemoglobin, can be quantitatively determined with a standard deviation of less than 10%. For the high concentration variants, such as variant S, the deviation is less than 1%.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.11501601107
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...