ISSN:
1612-1112
Keywords:
Capillary electrophoresis
;
Enzyme-catalyzed microreactions
;
Nicotinamide adenine dinucleotide
;
Quantitative analysis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary This work describes the quantitative analysis of incapillary enzyme-catalyzed microreactions using capillary electrophoresis. Migrating in a capillary under electrophoresis conditions, a plug of substrate and enzyme are injected separately, and allowed to react. Extent of reaction was subsequently determined by capillary electrophoresis. Two model systems were examined: the conversion of nicotinamide adenine dinucleotide (NAD) to nicotinamide adenine dinucleotide, reduced form (NADH) in the oxidation of glucose-6-phosphate (glc-6-p) to 6-phosphogluconate by glucose-6-phosphate dehydrogenase (G6PDH, EC 1.1.1.49), and the conversion of adenosine diphosphate (ADP) to adenosine monophosphate (AMP) by acid phosphatase (AP, EC 3.1.3.2.). These procedures illustrate the use of the capillary as a microreactor and the ease of quantitation of reaction products under conditions of electrophoresis. This technique offers a new and expeditious approach to analyzing enzymatic reactions on a microscale.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02466745
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