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Effectiveness of antineoplastic drugs on the proliferation of human mammary and ovarian carcinoma cells in monolayer culture (1985)

Hölzel, F. ; Albrecht, M. ; Simon, W. E. ; [et al.]

Springer

Journal of cancer research and clinical oncology 109 (1985), S. 217-226

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ISSN: 1432-1335

Keywords: Gynecological tumors ; Cellular heterogeneity in culture ; Chemosensitivity ; Proliferation assay ; Reproducibility

Source: Springer Online Journal Archives 1860-2000

Topics: Medicine

Notes: Summary Procedures for the in vitro determination of the drug-induced inhibition of mammary and ovarian carcinoma cell growth were established. In monolayer cultures derived from advanced tumors, separation of epithelial carcinoma cells from concomitant cells of fibroblast-like or mesothelial appearance was achieved by differential trypsinization. The carcinoma cell character of the stock cultures was verified by chromosome analyses showing a high degree of aneuploidy for the epitheloid cell lines and euploidy for cells of apparently mesenchymal origin. When cultured carcinoma cells were injected in nu/nu mice, the tissue and cell cultures obtained from the heterotransplantation tumors closely resembled the original tumors and cell cultures in morphology, karyotype, and expression of tumor markers. The action of carcinostatic drugs in the logarithmic phase of the carcinoma cell proliferation was tested by kinetics experiments in multiple experimental cultures. In cell proliferation assays based on cell counts the 50% inhibition dose (ID50) of the drug effects was determined from the dose-response curves. Comparison of the ID50s revealed highly differential effectiveness of the drugs examined. The inhibitory effects were reproducible, rendering the procedures used suitable for testing the chemosensitivity of newly explanted gynecological carcinoma cells by proliferation assays.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00390361

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Micropreparative system for enrichment of capillary GC effluents (1988)

Nitz, S. ; Drawert, F. ; Albrecht, M. ; [et al.]

Weinheim : Wiley-Blackwell

Journal of High Resolution Chromatography 11 (1988), S. 322-327

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ISSN: 0935-6304

Keywords: Preparative capillary GC ; Multidimensional GC ; Capillary techniques ; Enrichment ; Trace analysis ; Thermal desorption ; Chemistry ; Analytical Chemistry and Spectroscopy

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology

Notes: An automated system for preparative gas chromatography with capillary columns is described. The effluet from the capillary column is switched to the FID detector or to the traps by means of a Live-T switching device. The pneumatics is controlled by a microprocessor so that repetitive sampling can be performed over a period of days in order to enrich sufficient amount of material for NMR or other spectroscopic methods. The effluent containing the compounds is collected in glass tubes filled with column packing material (e.g. Chromosorb coated with 3% OV - 101, crosslinked). The trap temperatue can be adjusted from + 20°C to - 80°C, depending on the trapping material and volatility of trapped substances. The analysis of enriched substances or chromatographic fractions can be performed by thermal desorption of the same traps or by solven elution. The recovery of enriched substances is higher than 90%. High capacity and resolution for enrichment of trace components are obtained with the aid of a double column-double oven system. Examples of such applications are given.

Additional Material: 5 Ill.