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  • 1
    Electronic Resource
    Electronic Resource
    Capillary zone electrophoresis of aldoses, ketoses and uronic acids derivatized with ethylp-aminobenzoate (1992)
    Springer
    Chromatographia 34 (1992), S. 109-114 
    Details
    ISSN: 1612-1112
    Keywords: Capillary electrophoresis ; Carbohydrates ; Ethylp-aminobenzoate derivatives ; Borate complexes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A number of mono- and oligosaccharides derivatized with ethylp-aminobenzoate have been separated as their borate complexes by capillary zone electrophoresis, using a fused silica capillary containing 175 mM borate buffer, pH 10.5, as carrier. The derivatized carbohydrates were sufficiently separated within 17 min at an applied voltage of 25 kV. On-column UV monitoring allowed detection of these derivatives at the 10 fmol level, and quantification by the relative peak area method allowed reproducible determination of the saccharides at least in the concentration range of 0.5–20 mM in reaction solutions. This method has been applied to the determination of the monosaccharide compositions of polysaccharides extracted from Flos matricariae and Radix althaeae.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02276121
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Capillary zone electrophoretic analysis of carbohydrates by direct and indirect UV detection (1992)
    Springer
    Chromatographia 34 (1992), S. 308-316 
    Details
    ISSN: 1612-1112
    Keywords: Capillary electrophoresis ; Carbohydrates ; Precolumn derivatization ; Borate complexation ; Indirect UV detection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The analysis of carbohydrates has been always hampered by their lack of UV absorbance above 200 nm, which is an especially challenging problem in capillary electrophoresis due to the very small (nl) sample volumes injected. The introduction of 2-aminopyridine as derivatizing agent allows sensitive direct UV detection of saccharides in the fmol range. However, due to the requirement of the presence of a free aldehyde group only aldoses and uronic acids can be determined. This limitation was recently overcome by means of precolumn derivatization withp-aminobenzoic acid or ethylp-aminobenzoate, which permits the analysis of fructose with a lower mass detection limit of 0.3 and 0.14 pmol, respectively. The detection limits for aldoses were even as low as 15 and 7 fmol. A more universal approach is the use of indirect UV detection, which permits the analysis of carbohydrates, including (1–2)-linked disaccharides and aldonic acids, at the lower pmol level without the need for derivatization.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02268361
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    High-performance anion-exchange chromatography of carbohydrates using a new resin and pulsed amperometric detection (1995)
    Springer
    Chromatographia 41 (1995), S. 511-515 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Polymeric bonded phase ; Anion-exchange chromatography ; Pulsed amperometric detection ; Carbohydrates
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary This paper reports the results of a study on the use of a new polymer-based, strong anion-exchange, stationary phase for rapid and selective separation of carbohydrates and related compounds by high-pH, anion-exchange chromatography with pulsed amperometric detection. The new adsorbent has been obtained by direct nitration of 2.8 μm, spherical non-porous highly crosslinked, styrene-divinylbenzene copolymer beads, followed by reduction of superficially introduced nitro groups with nascent hydrogen and quaternization of the resultant amino groups with iodomethane. It is reported that by optimizing the ionic strength of the mobile phase, columns packed with the new anion-exchanger can be successfully employed to separate, either in isocratic or gradient elution mode, oligosaccharides, positional isomers of gluco-disaccharides, as well as uronic acids and sugar monophosphates.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02269712
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    Paper (German National Licenses)
    Fulltext
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