ISSN:
1432-2013
Keywords:
Indo-1
;
Flash photolysis
;
DM-nitrophen
;
Nd: YAG laser
;
Cardiac cells
;
293 cells
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract We have constructed a modular instrument to measure intracellular [Ca2+] ([Ca2+]i) in single isolated cells while simultaneously imposing step changes in [Ca2+]i using “caged Ca2+”. By combining the outputs of a xenon arc lamp with a frequency-tripled (Nd: YAG) laser, the instrument can operate with low maintained illumination to measure [Ca2+]i using a ratiometric Ca2+-sensitive fluorophore and also activate the release of Ca2+ from a caged-Ca2+ compound with a high energy pulse of ultraviolet light. This instrument is simple to assemble, introduces little electrical noise, provides a wide range of illumination power, produces only moderate photobleaching of the Ca2+ indicator and can be readily adapted to diverse cellular preparations. We demonstrate the use of this system to measure step changes in [Ca2+]i in adult rat ventricular myocytes and a human embryonic kidney cell line (293 cells) in culture.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00585957
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