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  • Cardiac muscle  (1)
  • DM-nitrophen  (1)
  • E-C coupling  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Propagation of excitation-contraction coupling into ventricular myocytes (1994)
    Springer
    Pflügers Archiv 428 (1994), S. 415-417 
    Details
    ISSN: 1432-2013
    Keywords: Heart ; Cardiac muscle ; Contraction ; E-C coupling ; Calcium channels ; Ryanodine receptors ; Intracellular calcium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract This paper examines the [Ca2+]i transient in isolated rat heart cells using a laser scanning confocal microscope and the calcium indicator fluo-3. We find that the depolarization-evoked [Ca2+]i transient is activated synchronously near the surface and in the middle of the heart cell with similar kinetics of activation. The time of rise of the transient did not depend on whether the sarcoplasmic reticulum (SR) Ca-release was abolished (by thapsigargin and ryanodine). The synchrony of activation and the similarity of levels of [Ca2+]i at the peripheral and deeper myoplasm (regardless of the availability of SR Ca-release) shows that sarcolemmal Ca channels and SR Ca-release channels are distributed throughout the rat heart cell and that the propagation of the action potential into the interior of the cell is rapid. In addition, the activation of calcium release from the SR by CICR is rapid (≪2 ms) when compared to the time-course of calcium influx via the sarcolemmal Ca channel.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00724526
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Measurement of intracellular Ca2+ concentration using Indo-1 during simultaneous flash photolysis to release Ca2+ from DM-nitrophen (1994)
    Springer
    Pflügers Archiv 427 (1994), S. 169-177 
    Details
    ISSN: 1432-2013
    Keywords: Indo-1 ; Flash photolysis ; DM-nitrophen ; Nd: YAG laser ; Cardiac cells ; 293 cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We have constructed a modular instrument to measure intracellular [Ca2+] ([Ca2+]i) in single isolated cells while simultaneously imposing step changes in [Ca2+]i using “caged Ca2+”. By combining the outputs of a xenon arc lamp with a frequency-tripled (Nd: YAG) laser, the instrument can operate with low maintained illumination to measure [Ca2+]i using a ratiometric Ca2+-sensitive fluorophore and also activate the release of Ca2+ from a caged-Ca2+ compound with a high energy pulse of ultraviolet light. This instrument is simple to assemble, introduces little electrical noise, provides a wide range of illumination power, produces only moderate photobleaching of the Ca2+ indicator and can be readily adapted to diverse cellular preparations. We demonstrate the use of this system to measure step changes in [Ca2+]i in adult rat ventricular myocytes and a human embryonic kidney cell line (293 cells) in culture.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00585957
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
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