ISSN:
1432-203X
Keywords:
Key words Embryogenic suspension cultures
;
β-Glucuronidase
;
Agrobacterium tumefaciens
;
Cassava
;
Paromomycin
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A protocol was developed for Agrobacterium-mediated transformation of embryogenic suspension cultures of cassava. The bacterial strain ABI containing the binary vector pMON977 with the nptII gene as selectable marker and an intron-interrupted uidA gene (encoding β-glucuronidase) as visible marker was used for the experiments. Selection of transformed tissue with paromomycin resulted in the establishment of antibiotic-resistant, β-glucuronidase-expressing lines of friable embryogenic callus, from which embryos and subsequently plants were regenerated. Southern blot analysis demonstrated stable integration of the uidA gene into the cassava genome in five lines of transformed embryogenic suspension cultures and in two plant lines.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002990050492
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