ISSN:
1432-072X
Keywords:
Acetohydroxy Acid Synthetase (pH 6.5)
;
Bacillus subtilis
;
Isoenzymes
;
Catabolic and Anabolic Isoenzymes
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Acetohydroxyacid synthetase activity of crude extracts ofBacillus subtilis grown in pH 7.0 minimal medium has a pH optimum of 7.5. However, the activity of extracts of cells grown in minimal medium of pH 6.0 shows a pH optimum of 6.5. Acetate or propionate induces formation of the pH 6.5 activity. Hydroxyapatite chromatography of a crude extract of cells grown in pH 7.0 medium shows one major and one minor peak of enzymatic activity. Both peaks have a pH optimum of 7.5–8.0. However, chromatography of an extract of cells grown in the presence of acetate reveals three peaks of activity: one major peak with a pH optimum of 6.5 and two minor peaks both having a pH optimum of 7.5–8.0.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00590182
Permalink
