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  • 1
    ISSN: 1432-1912
    Keywords: Dopamine ; Secretion ; Adrenal Medulla ; Catecholamine ; Biosynthesis-Rate ; Dopamin ; Sekretion ; Nebennierenmark ; Catecholamine ; Biosyntheserate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Cats and rabbits were injected intravenously with3H-tyrosine and the radioactivity of the catecholamines in adrenal glands and adrenal blood was measured up to 60 min after injection. Adrenal blood was collected in a cava pocket. In addition the specific activity of free3H-tyrosine in blood plasma was measured as a function of time. Radioactive dopamine could be demonstrated in venous blood from adrenals of cat and rabbit by different methods (column chromatography with Dowex 50, paper chromatography, high voltage paper electrophoresis). On the other hand arterial blood flowing to the adrenals contained no3H-dopamine. During the 30 min after the injection of3H-tyrosine cat adrenals secreted about 14% of the synthesized radioactive catecholamines as3H-dopamine and 8% as3H-noradrenaline. In contrast to this the adrenals of rabbits secreted not more than 3% as3H-dopamine and about 20% as3H-noradrenaline. The catecholamine biosynthesis rate of the adrenals and their dopamine resting secretion was estimated from the specific activity of3H-tyrosine in blood plasma and the radioactivity of catecholamines in adrenal glands and adrenal blood. In cats two adrenals synthesized 0.75 mμMol/min catecholamine per kg body weight and in rabbits 0.074 mμMol/min/kg body wt. respectively. The dopamine resting secretion in cats was about 0.08 mμMol/min and in rabbits about 0.002 mμMol/min.
    Type of Medium: Electronic Resource
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