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Indole alkaloid production by hairy root cultures of Catharanthus roseus (1989)

Toivonen, L. ; Balsevich, J. ; Kurz, W. G. W.

Springer

Plant cell, tissue and organ culture 18 (1989), S. 79-93

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ISSN: 1573-5044

Keywords: Agrobacterium rhizogenes ; Catharanthus roseus ; hairy root cultures ; indole alkaloids

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract Hairy root cultures of Catharanthus roseus were established by infection with six different Agrobacterium rhizogenes strains. Two plant varieties were used and found to exhibit significantly different responses to infection. Forty-seven hairy root clones derived from normal plants and two derived from the flowerless variety were screened for their growth and indole alkaloid production. The growth rate and morphological appearance showed wide variations between the clones. The alkaloid spectra observed were qualitatively but not quantitatively very similar to that of the corresponding normal plant roots. No vindoline or deacetyltransferase activity could be detected in any of the cultures studied. O-acetylval-lesamine, an alkaloid which has not been previously observed in C. roseus was identified from extracts of hairy root clone No. 8. Two root clones were examined for their growth and alkaloid accumulation during a 26-day culture period. Alkaloid accumulation parallelled growth in both clones with ca. 2 mg ajmalicine and catharanthine per g dry weight being observed.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00033467

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Studies on the optimization of growth and indole alkaloid production by hairy root cultures of Catharanthus roseus (1991)

Toivonen, L. ; Ojala, M. ; Kauppinen, V.

New York, NY [u.a.] : Wiley-Blackwell

Biotechnology and Bioengineering 37 (1991), S. 673-680

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ISSN: 0006-3592

Keywords: indole alkaloid production ; Catharanthus roseus ; Agrobacterium rhizogenes ; hairy root cultures ; Chemistry ; Biochemistry and Biotechnology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology

Notes: Catharanthus roseus hairy root cultures, genetically transformed with Agrobacterium rhizogenes, produce a wide variety of indole alkaloids. The effect of sucrose, phosphate, nitrate, and ammonia concentrations on growth and indole alkaloid production of C. roseus hairy root cultures were studied by using statistical experimental designs and linear regression analysis. Contradictory effects of these nutrients on growth and indole alkaloid production were found. The maximal growth was obtained by having 77. 8 mg NaH2PO4 · H2O/L and 1. 311 g KNO3/L in the medium, whereas the specific production of alkaloids was highest at the lowest levels of all the nutrients studied. The maximal dry weight was obtained with high values of sucrose and ammonia, but clear optimum concentrations could not be found. When having enough nutrients to support reasonable growth, it appeared difficult to affect the specific alkaloid production rates considerably. The growth (dry wt.) with the optimized nutrient concentrations in the medium was more than 50% better than in the control medium with about the same alkaloid production.

Additional Material: 7 Ill.