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Simultaneous determination of cefoxitin, cefuroxime, cephalexin and cephaloridine in plasma using HPLC and a column-switching technique (1990)

Lee, Y. J. ; Lee, H. S.

Springer

Chromatographia 30 (1990), S. 80-84

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ISSN: 1612-1112

Keywords: Column liquid chromatography ; Cephalosporins ; Column switching in HPLC ; On-line plasma sample clean-up

Source: Springer Online Journal Archives 1860-2000

Topics: Chemistry and Pharmacology

Notes: Summary A new high performance liquid chromatographic method was developed using a column-switching technique for the simultaneous determination of cephalexin, cefuroxime, cefoxitin and cephaloridine in plasma. The plasma samples were injected onto a precolumn packed with Corasil RP C18 (37–50 μm) after simple dilution with an internal standard solution in 0.01 M acetate buffer (pH 3.5). Polar plasma components were washed out using 0.01 M acetate buffer (pH 3.5). After valve switching, the concentrated drugs were desorbed in back-flush mode and separated on a Partisil ODS-3 column using acetonitrile in 0.02 M acetate buffer (pH 4.3) (15∶85, v/v) as the mobile phase. The method showed excellent precision with good sensitivity and speed with a detection limit of 0.5 μg/ml. The total analysis time per sample was less than 25 min, and the mean coefficients of variation for intra- and inter-assay were both less than 4.9 %. This method has been successfully applied to plasma from rats after subcutaneous injection of cefuroxime.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF02270453

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A review of recent observations concerning the synaptic organization of the basilar pontine nuclei (1988)

Mihailoff, G. A. ; Kosinski, R. J. ; Border, B. G. ; [et al.]

New York, NY : Wiley-Blackwell

Journal of Electron Microscopy Technique 10 (1988), S. 229-246

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ISSN: 0741-0581

Keywords: Cerebellum ; Cerebral cortex ; Dorsal column nuclei ; Cerebellar nuclei ; Life and Medical Sciences ; Cell & Developmental Biology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Natural Sciences in General

Notes: Ultrastructural studies are described that have identified in the basilar pontine nuclei (BPN), the synaptic boutons formed by the corticopontine, cerebellopontine, tectopontine, and dorsal column nucleipontine afferent projection systems. In addition, immunocytochemical studies visualized neuronal somata, dendrites, and synaptic boutons that contain immunoreactivity for GABA or the synthesizing enzyme glutamic acid decarboxylase (GAD).Based upon differences in the mode of degeneration and postsynaptic locus of degenerative synaptic boutons in the BPN, it is suggested that two types of cortical neurons and three classes of deep cerebellar nuclear cells project to the BPN. For similar reasons, it appears that two types of neurons in the dorsal column nuclei project to the BPN while only one type of afferent synaptic bouton takes origin from the superior colliculus. Furthermore it appears that the population of BPN neurons projecting to the paramedian lobule receives convergent inputs from the cutaneous periphery and the corresponding region of sensorimotor cortex. Studies employing GAD immunohistochemistry indicate that GABA-ergic neurons and axon terminals are present in the BPN and thus support the suggestion that a local inhibitory interneuron is present within the BPN. Taken together these observations suggest that basilar pontine neurons might play a more active role in the integration of various types of information destined for the cerebellar cortex than has previously been recognized.

Additional Material: 5 Ill.