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Candida albicans ALS3 and insights into the nature of the ALS gene family (1998)

Hoyer, L. L. ; Payne, Tracie L. ; Bell, M. ; [et al.]

Springer

Current genetics 33 (1998), S. 451-459

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ISSN: 1432-0983

Keywords: Key wordsCandida albicans ; Gene family ; Hyphal-specific ; Differential gene regulation

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract The ALS1 (agglutinin-like sequence) gene of Candida albicans encodes a protein similar to alpha-agglutinin, a cell-surface adhesion glycoprotein of Saccharomyces cerevisiae (Hoyer et al. 1995). A central domain of a tandemly repeated 108-bp sequence is found in the ALS1 coding region. This tandem-repeat motif hybridizes to multiple C. albicans genomic DNA fragments, indicating the possibility of other ALS1-like genes in C. albicans (Hoyer et al. 1995). To determine if these fragments constitute a gene family, tandem-repeat-hybridizing genomic fragments were isolated from a fosmid library by PCR screening using primers based on the consensus tandem-repeat sequence of ALS1 (Hoyer et al. 1995). One group of fosmids, designated ALS3, encodes a gene with 81% identity to ALS1. The sequences of ALS1 and ALS3 are most conserved in the tandem-repeat domain and in the region 5′ of the tandem repeats. Northern-blot analysis using unique probes from the 3′ end of each gene demonstrated that ALS1 expression varies, depending on which C. albicans strain is examined, and that ALS3 is hyphal-specific. Both genes are found in a variety of C. albicans and C. stellatoidea strains examined. The predicted Als1p and Als3p exhibit features suggesting that both are cell-surface glycoproteins. Southern blots probed with conserved sequences from the region 5′ of the tandem repeats suggest that other ALS-like sequences are present in the C. albicans genome and that the ALS family may be larger than originally estimated.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/s002940050359

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Monocyte kinetics: Observations after pulse labelingSupported by a research grant from the Medical Research Council of Canada. (1968)

Whitelaw, D. M. ; Bell, M. F. ; Batho, H. F.

New York, NY [u.a.] : Wiley-Blackwell

Journal of Cellular Physiology 72 (1968), S. 65-71

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ISSN: 0021-9541

Keywords: Life and Medical Sciences ; Cell & Developmental Biology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Medicine

Notes: Because monocytes and their precursors cannot be recognized with certainty in tissues, an approach to the study of monocyte kinetics was made through examination of the peripheral blood. Injection of a single pulse of tritiated thymidine into rats resulted in the appearance of labeled monocytes identified as circulating peroxidase-positive mononuclear cells. The increase in the percent of labeled cells and in the mean grain count per cell followed a course described by a mathematical model with a generation time of 21 hours and a DNA synthesis time of 12.5 hours. The generation and synthesis times appear to be very uniform for the monocyte so that the phasing of cells represented by the uptake of label could be followed for more than two generations, a property not shared by neutrophils or lymphocytes. Monocytes appear in the circulation within eight hours of DNA synthesis.

Additional Material: 4 Ill.