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  • 1
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 113 (1982), S. 213-217 
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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  • 2
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 226 (1990), S. 471-480 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The distribution and cellular localization of the glycoprotein laminin were investigated by light and electron microscopic immunocytochemistry in the adult murine pituitary gland. Immunoblots confirmed that laminin was the only protein in the pituitary gland of the adult male mouse to react with antilaminin serum. Laminin immunoreactivity was demonstrated at the light microscopic level simultaneously with that of β-follicle stimulating hormone (β-FSH) and β-luteinizing hormone (β-LH). In addition to its distribution is basal laminae, laminin immunoreactivity was coincidently expressed in gonadotrophs with the immunoreactivities of β-FSH and β-LH. Electron microscopic immunocytochemistry was employed on aldehyde-fixed sections embedded in L.R. White. Sites of binding of primary antisera to laminin were identified with affinity-purified secondary antisera directly coupled to 20 nm particles of colloidal gold. Three antisera recognizing laminin were compared and found to result in an identical pattern of immunoreactivity. Laminin was found extracellulary only in formed basal laminae in all three lobes of the pituitary and was not found in extracellular matrices of connective tissue. Laminin immunoreactivity was also found intracellularly in gonadotrophs but in none of the other endocrine or non-endocrine cells of the anterior lobe. Within gonadotrophs, only secretory granules were labeled. The majority, but not all, secretory granules were labeled in each of the gonadotrophs examined, and the proportion of granules labeled with laminin could not be increased by doubling the concentration of anti-laminin serum. Laminin immunocreactivity segregated with the subset of secretory granules containing β-FSH. In contrast, laminin immunoreactivity was absent in the smaller subset of secretory granules that contain serotonin. No secretory granules were labeled within the endocrine cells of the intermediate lobe, nor within secretory granules of neural elements in the posterior lobe.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 15 (1980), S. 470-476 
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: An algorithm is given to discretize a polygon or curved surface into triangular arrays. The method first involves the manual division of the array into quadrilaterals with specified numbers on rows and columns. The individual points are then filled in automatically and elements are automatically generated. A point of particular importance is that the number of columns or rows may be made to change. A subroutine is given to perform this task.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    BioEssays 15 (1993), S. 651-658 
    ISSN: 0265-9247
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The eukaryotic cell is partitioned by membranes into spatially and functionally discrete subcellular organelles. In addition, the cytoplasm itself is partitioned into discrete subregions that carry out specific functions. Such compartmentation can be achieved by localizing proteins and RNAs to different subcellular regions. This review will focus on localized RNAs, with a particular emphasis on RNA localization mechanisms and on the possible biological functions of localization of these RNAs. In recent years, an increasing number of localized RNAs have been identified in a variety of cell types among many animal species. Emphasis here will be on localized RNAs in the most intensively studied systems - Drosophila and Xenopus eggs and early embryos.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Chichester : Wiley-Blackwell
    International Journal for Numerical Methods in Fluids 10 (1990), S. 925-945 
    ISSN: 0271-2091
    Keywords: Bi-conjugate gradient ; Conjugate gradient ; Conjugate gradient squared ; Finite elements ; Flow simulation ; Preconditioning ; Vector processor ; Frontal solver ; Navier-Stokes ; Boussinesq approximation Newton method ; Picard iteration ; Galerkin method ; Petrov-Galerkin ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: A series of numerical experiments on the Cray XMP/48 and on the Cray 2 investigate the robustness and economy of direct and unsymmetric conjugate gradient (CG) type methods for the solution of matrix systems arising from a 3D FEM discretization of fluid flow problems. Computations on a Boussinesq flow model problem with either ILU preconditioned or unpreconditioned unsymmetric CG methods are presented. Such experiments seem to indicate that the unpreconditioned BICG method is robust for moderately non-linear incompressible Navier-Stokes FEM discretizations and that the ILU preconditioned BICG method is very robust and more economic than an unsymmetric frontal solver when the generous memory of the Cray 2 is exploited to store both the matrix and its preconditioner. We cover some of the programming aspects of direct and iterative methods on a supercomputer and find that direct methods have advantages: the crucial CPU-consuming area of code is compact but overwhelming, and its percentage of total CPU usage is independent of the spectral properties of the matrix involved. An optimal implementation of the unsymmetric CG method is more difficult because its work is related to the spectral distribution of the matrix considered and because there is no single portion of the code that overwhelmingly dominates the CPU usuage.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
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