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Mapping of rRNA genes by integration of hybrid plasmids in Schizosaccharomyces pombe (1984)

Toda, Takashi ; Nakaseko, Yukinobu ; Niwa, Osami ; [et al.]

Springer

Current genetics 8 (1984), S. 93-97

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ISSN: 1432-0983

Keywords: Integration vector ; Nucleolar DNA ; rRNA gene mapping ; Schizosaccharomyces pombe

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Summary The major rRNA genes of the fission yeast Schizosaccharomyces pombe were mapped on chromosome III by plasmid integration. The integration vector YIp33 containing S. cerevisiae LEU2 gene was combined with the S. pombe rDNA. Since LEU2 complements S. pombe leu1 deficiency, it could be used as the genetic marker for integration. The 10.4 kb rDNA repeat contained ARS sequence, and therefore 2.4 kb and 0.7 kb subfragments not containing ARS were subcloned into YIp33 and transformed leu1 S. pombe cells to Leu+. Genetic analyses of the transformants indicated that the integrated rDNA resides in the long arm of the shortest chromosome III, tightly linked to ade5 (1.4 cM). This result is consistent with our previous finding that the DAPI-stained smallest chromosomes were associated with the nucleolus (Umesono et al. 1983).

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00420224

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A telomerase mutant defective in sister chromatid separation at mitosis (1997)

Nakaseko, Yukinobu ; Yanagida, Mitsuhiro

New York, NY : Wiley-Blackwell

BioEssays 19 (1997), S. 557-559

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ISSN: 0265-9247

Keywords: Life and Medical Sciences ; Cell & Developmental Biology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Medicine

Notes: The telomere is a functional domain of the chromosome, located at the extreme ends, and is essential for normal chromosome stability. Chromosomes lacking telomeres are inherited improperly, and mutations in the telomeric repeat sequences are thought to lead to senescence and possibly to cancer. The molecular mechanisms maintaining chromosomes by telomeres, however, have been unclear. Results recently reported by Kirk et al.(1) offer an insight into new telomerase function. They have identified a novel telomerase mutation that blocks sister chromatid separation in mitosis.

Additional Material: 1 Ill.