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Hypochlorite generation on Ru–Pt binary oxide for treatment of dye wastewater (2000)

Yang, C.-H. ; Lee, C.-C. ; Wen, T.-C.

Springer

Journal of applied electrochemistry 30 (2000), S. 1043-1051

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ISSN: 1572-8838

Keywords: dye wastewater ; hypochlorite production ; Ruthenium–Platinum binary oxides

Source: Springer Online Journal Archives 1860-2000

Topics: Chemistry and Pharmacology , Electrical Engineering, Measurement and Control Technology

Notes: Abstract Ruthenium–platinum binary oxides [(Ru + Pt)O x ] were coated on titanium substrates by thermal decomposition. The surface morphologies and elemental analyses of these electrodes were examined by means of scanning electron microscopy. The electrochemical behaviour was characterized by cyclic voltammetry (CV) and linear scanning voltammetry (LSV). The effects of electrolyte conditions on the current efficiency (CE) of hypochlorite production on binary (Ru + Pt)O x electrodes and the treatment of a high salt-containing dye wastewater using this hypochlorite were also investigated. The highest CE for hypochlorite production occurred on the RP1 (20 mol% Pt in precursor) electrode. The major factors influencing CE for hypochlorite production were the electrolyte flow rate, current density and chloride ion (C1−) concentration. The RP1 electrode exhibited the best removal of organics and chromophoric groups in the dye wastewater. On this electrode, better removal of organics and chromophoric groups was obtained at 300 mA cm−2. The colour of black–red dye wastewater became light yellow when a charge of 13.2 A h was passed while the COD of the wastewater decreased from 10 500 to 1250 mg L−1.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1023/A:1004038503410

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The effect of vesical outlet obstruction on the protein secondary structure of the mucosa and serosa in rabbit bladder wall (1994)

Lin, S. Y. ; Liang, R. C. ; Yang, C. H. ; [et al.]

Springer

Urological research 22 (1994), S. 205-208

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ISSN: 1434-0879

Keywords: Bladder outlet obstruction ; Mucosa ; Serosa ; Rabbit ; FT-IR ; ATR ; Protein secondary structure

Source: Springer Online Journal Archives 1860-2000

Topics: Medicine

Notes: Abstract The biochemical composition of physiologically moist mucosa and serosa of rabbit bladder before and after bladder outlet obstruction was determined by means of FT-IR spectroscopy with the ATR method and second-derivative analysis. A predominantly β-sheet structure was found in the amide I band for mucosa and serosa before and after obstruction, but the random coil structure increased in both obstructed bladder samples. However, the major β-sheet structure associated with some α-helical structure in the amide II band of mucosa and serosa for non-obstructed bladder changed into a predominanly α-helical structure after bladder obstruction. The obstructed bladder serosa was more pronounced. The amount of glycoproteins doubled in the obstructed bladder serosa, but did not change in the bladder mucosa.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00541893

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The use of multiplane transesophageal echocardiography to evaluate residual patent ductus arteriosus during video-assisted thoracoscopy in adults (1999)

Ho, A. C. Y. ; Tan, P. P. C. ; Yang, M.-W. ; [et al.]

Springer

Surgical endoscopy and other interventional techniques 13 (1999), S. 975-979

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ISSN: 1432-2218

Keywords: Key words: Patent ductus arteriosus — Transesophageal echocardiography — Video-assisted thoracoscopic surgery

Source: Springer Online Journal Archives 1860-2000

Topics: Medicine

Notes: Abstract Background: Video-assisted thoracoscopic surgery (VATS) has emerged as an innovative and popular procedure for interruption of patent ductus arteriosus (PDA), while intraoperative transesophageal echocardiography (TEE) has proven to be an effective monitor in the evaluation of residual patency. Previous reports on the adequacy of surgical interruption of PDA under VATS and TEE are available for pediatric patients, but only limited information is available for adults with PDA. Materials and methods: Between August 1995 and October 1997, we monitored 35 adult patients undergoing PDA interruption via VATS with Hewlett-Packard color Doppler multiplane TEE throughout the procedure. The average PDA diameter was 10.2 ± 1.8 mm. All the PDA were completely ligated. Results: Thirty-two patients showed no ductal flow after double ligation. In the other three patients, residual flow was detected intraoperatively after double ligation, but it was quickly abolished by the third ligation. One patient showed faint ductal flow by transthoracic echocardiography at postoperative follow-up, but no reintervention was needed. Conclusions: Our study showed that, with the refinement of adult PDA interruption via VATS, intraoperative multiplane TEE provides higher resolution for direct evaluation of the entire course of PDA ligation without interrupting the surgical procedure and minimizes the incidence of complications.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/s004649901150

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Altered molecular properties of tubulin in a multidrug-resistant variant of Chinese hamster cells selected for resistance to vinca alkaloids (1988)

Pain, J. ; Sirotnak, F. M. ; Barrueco, J. R. ; [et al.]

New York, NY [u.a.] : Wiley-Blackwell

Journal of Cellular Physiology 136 (1988), S. 341-347

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ISSN: 0021-9541

Keywords: Life and Medical Sciences ; Cell & Developmental Biology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Medicine

Notes: The basis for the markedly altered intracellular binding of [3H]vincristine in a multidrug-resistant variant (DC-3F/VCRd-5L) of Chinese hamster lung cells (DC-3F) was investigated. Binding of [3H]vincristine by protein in cytosol derived from each cell type exhibited a differing requirement for GTP in MgCl2 containing buffer of low-ionic strength. Binding of [3H]vincristine occurred to cytosolic protein derived from both variant and parental DC-3F cells, but after removal of GTP, binding only occurred to cytosolic protein from parental cells regardless of the presence of added GTP. Although binding by cytosolic protein from parental DC-3F cells did not require GTP, the addition of 0.1 mM GTP increased by two-fold the rate and extent of binding. When cytosol from variant and parental DC-3F cells was incubated with low concentrations of [3H]vincristine in high-ionic strength buffer and analyzed by molecular-sieve HPLC, most of the protein binding [3H]vincristine in parentally derived cytosol eluted as Mr 110,000-115,000 daltons, corresponding to that for dimeric tubulin. The same binding species was not detected in cytosol derived from variant cells. However, these same fractions derived with both parental and variant cytosols contained tubulin as shown by SDS-PAGE and immunoblotting. A smaller peak of [3H]vincristine binding and an amount of tubulin equal to that found in later fractions were found in the void volume during the same HPLC elution runs with cytosol from both variant and parental DC-3F cells. Evidence was also obtained for differences between parental and variant DC-3F cells in β-tubulin isoforms following isoelectric focusing and immunoblotting. Parental-cell cytosol contains a single isoform of β-tubulin. However, in variant cell cytosol the same isoform and, in addition, three more basic isoforms were found. These alterations in [3H]vincristine binding and in isoform compositions of β-tubulin in variant versus parental DC-3F cells may have importance in regard to vincristine resistance in DC-3F cells.

Additional Material: 6 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/jcp.1041360218

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Markedly altered membrane transport and intracellular binding of vincristine in multidrug-resistant Chinese hamster cells selected for resistance to vinca alkaloids (1986)

Sirotnak, F. M. ; Yang, C.-H. ; Mines, L. S. ; [et al.]

New York, NY [u.a.] : Wiley-Blackwell

Journal of Cellular Physiology 126 (1986), S. 266-274

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ISSN: 0021-9541

Keywords: Life and Medical Sciences ; Cell & Developmental Biology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Medicine

Notes: Studies of a multidrug-resistant variant (DC-3F/VCRd-5L) of Chinese hamster lung cells selected for resistance to vinca alkaloids revealed marked alterations in transport and intracellular binding of [3H]vincristine compared to parental DC-3F cells. Influx of [3H]vincristine in DC-3F cells appears to be an equilibrating, but mediated, process. Although saturation kinetics for [3H]vincristine influx were not demonstrated, an extremely high temperature-dependence (Q1027-37°C = 5-6) and trans-inhibition of influx following preloading of cells with nonradioactive vincristine argue in favor of a carrier-mediated process. Efflux of [3H]vincristine from parental cells conformed to first-order kinetics (t1/2 37° = 3.6 ± 0.4) and exhibited a lower temperature-dependence (Q10 27-37°C = 3-3.5) than influx. In variant vs. parental cells, influx of [3H]vincristine was reduced 24-fold and efflux was increased twofold, accounting for the large (approximately 48-fold) reduction in steady-state level of exchangeable drug accumulating in variant cells. Otherwise, transport of [3H] vincristine in these cells showed characteristics similar to parental DC 3F cells. Also, the rate and amount of intracellular binding of [3H]vincristine in variant cells was almost 40-fold lower than in parental cells. These alterations in influx and efflux of [3H]vincristine and its intracellular binding appear to account, at least to a major extent, for the high level of resistance (2,750-fold) of this variant to vinca alkaloids. In contrast, cross-resistance of this variant to daunomycin (178-fold) could be explained only minimally by a transport alteration. Only a two-fold increase in efflux of [3H]daunomycin was demonstrated in variant vs. parental cells along with some decrease in intra-cellular binding. Influx of [3 H]daunomycin was unaltered. In view of these results, we conclude that these two agents most likely do not share the same route for entry in these cells but might share the same efflux route.

Additional Material: 9 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/jcp.1041260217

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