ISSN:
1432-0983
Keywords:
Cell Cycle
;
Phosphoprotein
;
Schizosaccharomyces pombe
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary The 34 kilodalton protein product (p34) of the cdc2 + cell cycle control gene of Schizosaccharomyces pombe was expressed in bacteria. Monoclonal antibodies raised against this protein are capable of immunoprecipitating p34dc2 from yeast lysates. Immunoprecipitates of [35S]methionine- and [32P]orthophosphate-labeled p34cdc2 were analyzed by two-dimensional gel electrophoresis. The cdc2 + gene product is homogeneous in size but resolves into seven species of differing charge. At least four of these species are phosphorylated. Phosphoamino acid analysis reveals that phosphorylation occurs mainly on threonine residues. The pattern of p34 phosphorylation is unaltered at the nonpermissive temperature in strains carrying temperature sensitive alleles of wee1-50 and ran1-114 or in a strain over-producing the ran1 + gene product.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00376743
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