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  • Chemistry  (32)
  • Lolium perenne  (6)
  • Lycopersicon esculentum  (4)
  • Cell culture  (3)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Urological research 5 (1977), S. 207-210 
    ISSN: 1434-0879
    Keywords: Cell culture ; Prostate ; Collagenase ; Epithelial
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A procedure is described which yields a significant percentage of long-term mixed cell cultures of human prostatic tissue. Attempts were made to suppress the proliferation of stromal fibroblasts and to characterize the cultured cells as those of prostatic origin. The problems associated with establishing epithelial cell lines are discussed.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-2048
    Keywords: Antimetabolites ; Cell culture ; Enzyme induction ; Molybdenum ; Nitrate reductase ; Rosa, Paul's Scarlet
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Induction of nitrate reductase (EC 1.6.6.1) activity was measured in Paul's Scarlet rose cell suspensions cultured in media containing nitrate (NO 3 - ) or urea (U) as nitrogen source, and with (+Mo) or without molybdenum (-Mo). There was a lag of 30 min during induction by NO 3 - in +Mo cultures but no lag occurred during induction after adding Mo to NO 3 - -Mo or to U-Mo cultures preincubated with NO 3 - . Actinomycin D, cycloheximide, and puromycin completely blocked induction by NO 3 - , but had no effect on the initial rate of induction by Mo. Cycloheximide and puromycin blocked induction by NO 3 - more quickly than actinomycin D. Induction by NO 3 - appeared to involve mRNA-dependent synthesis of apoprotein followed by rapid activation with molybdenum in intact cells independently of protein synthesis. Nitrate-induced apoprotein appeared less stable than the holoenzyme. When induced by NO 3 - in the absence of Mo, apoprotein concentration was about half the amount of maximally induced nitrate reductase. Cycloheximide stabilised preformed nitrate reductase which disappeared steadily in the presence of puromycin. Apoprotein was not stabilised by either antimetabolite.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-2048
    Keywords: Rose ; Paul's Scarlet ; Cell culture ; Nitrate reductase ; Molybdenum ; Enzyme induction ; Enzyme assay
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Growth and nitrate reductase activity were measured in Paul's Scarlet rose cell suspensions, cultured in media purified from molybdenum and containing nitrate or urea as sole nitrogen source with or without added Mo. Urea could replace nitrate to yield 80% of the fresh weight in nitrate medium. Nitrate reductase activities were compared by in vivo and in vitro assays. The latter varied due to inactivation during extraction. Compared with activities in cells in complete NO3 - medium, activity in NO3 --Mo cells was reduced to 30% and, in urea-grown cells, to trace amounts. Increases in nitrate reductase activity were found when NO3 - alone was added to NO3 - or urea+Mo cultures. In NO3 --Mo cultures, Mo alone or with NO3 - caused a similar increase in activity, whereas urea-Mo cultures required both NO3 - and Mo for enzyme induction.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 65 (1983), S. 149-156 
    ISSN: 1432-2242
    Keywords: Lolium perenne ; Meiosis ; Chiasma distribution ; U-type exchange
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In two contrasting types of S5 inbred Lolium perenne a correlation has been found between the distribution pattern of chiasmata within bivalents and that of meiotic U-type exchange. The relationship between the two processes is discussed, in terms of inbreeding effects upon chiasma redistribution, and mention is also made of some other types of chromosome breakages which arise following enforced self-pollination.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 64 (1983), S. 137-145 
    ISSN: 1432-2242
    Keywords: Lolium perenne ; Inbred lines ; Meiosis ; Chiasma distribution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary An analysis of the patterns of chiasma distribution within bivalents, in a number of inbred lines of Lolium perenne, reveals a striking potential for variation in this character, as well as a polygenic genetic basis for its control. In parent plants the chiasmata have a regular pattern of localisation which restricts their formation to the ends of the chromosome arms: this strict localisation is progressively lost with inbreeding and novel patterns of distribution are found where chiasmata occur in more interstitial and proximal regions. The variation is continuous and originates from genotypic differences between the different parent plants, rather than from segregation among families deriving from individual parents.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 81 (1991), S. 321-326 
    ISSN: 1432-2242
    Keywords: Lycopersicon esculentum ; Salt tolerance ; Seed germination ; Maternal effects ; Tomato improvement ; Gene action
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The salt-tolerant cultivated tomato (Lycopersicon esculentum) accession, ‘PI174263’, and a sensitive cv, ‘UCT5’, were crossed to develop reciprocal F1, F2 and BC1 populations for genetic analysis of salt tolerance in tomatoes during seed germination. Variation was partitioned into embryo, endosperm and maternal (testa and cytoplasmic) components. Generation means analysis indicated that there were no significant embryo (additive, dominance or epistatic) effects on germination performance under salt stress. Highly significant endosperm additive and testa dominance effects were detected. The proportion of the total variance explained by the model containing these two components was R2=98.2%. Variance component analysis indicated a large genetic variance with additive gene action as the predominant component. Furhter inspection indicated that this variance was attributable to endosperm additive effects on germinability under salt stress. Narrow-sense heritability was estimated as moderately high. Implications for breeding procedures are discussed.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 62 (1982), S. 177-183 
    ISSN: 1432-2242
    Keywords: Lolium perenne ; Inbred lines ; Meiosis ; Chiasmata
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A number of inbred lines of Lolium perenne have been developed as far as the fifth inbred generation and are being used for basic studies on the cytogenetics of the species. An analysis on variation in the pattern of chiasma frequency and distribution, including the parent plants and all generations down to the S5, reveals that the effect of inbreeding is to reduce chiasma frequency and to increase both the cell and bivalent variances. Evidence is presented for a genetic basis of polygenic control of chiasma formation and distribution, and a model is suggested for control over the three related components of variation which involves a two-stage level of regulation.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 74 (1987), S. 233-241 
    ISSN: 1432-2242
    Keywords: Lolium perenne ; Chromosome doubling ; Colchicine-induced variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A number of diploid inbred lines of Lolium perenne were treated with colchicine at the early seedling stage to induce chromosome doubling. In each inbred line the colchicine-treated undoubled diploids were kept as controls, as well as the normal untreated diploids. Comparisons of vegetative growth and development, involving the three treatments within each line, revealed that colchicine treatment of seedlings has long-lasting effects upon plant growth and development independent of chromosome doubling, and that for certain characteristics the effects of chromosome doubling are confounded with other effects of the treatment used to produce tetraploids. This colchicine induced variation in the diploids is transmitted through the seed generations in at least one of the inbred lines. The variation appears to be non-random and also shows a strong genotypic component. In so far as the effects of chromosome doubling could be determined, they showed the usual gigas response but were again strongly influenced by genotype.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Euphytica 40 (1989), S. 187-191 
    ISSN: 1573-5060
    Keywords: Lycopersicon esculentum ; tomato ; Pyrenochaeta lycopersici ; corky root ; brown root rot ; resistance ; greenhouse screening method
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A greenhouse screening method for corky root (Pyrenochaeta lycopersici) resistance in the tomato (Lycopersicon esculentum) is described in detail. In determining the reliability of the method, known resistance sources within wild asccessions, commerical cultivars and breeding lines were evaluated for resistance in fields naturally infested with corky root and their response compared under greenhouse conditions in soil heavily infested with corky root and artifically maintained at temperatures between 10–13°C. The procedure developed can be used year round and improves the efficiency of recovering resistant progeny in large segregrating populations.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1573-5060
    Keywords: Lycopersicon esculentum ; tomato ; cold tolerance ; seed germination ; genetic analysis ; missing data ; response-time data
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In studies to determine the inheritance of response-time traits, such as time to seed germination, some viable individuals may fail to respond during an experiment. If these right-censored observations are ignored, sample means and variances will be underestimated. This is illustrated using data from time to seed germination at 9°C for Lycopersicon esculentum (Mill.) fast germinating PI 120256, slow-germinating T3 and their reciprocal F1, F2 and backcross progeny. This paper presents methods to detect and to accommodate right-censored data in generation means analysis. Genetic interpretations derived from corrected and uncorrected estimates of generation means and variances are compared. Correction for right-censoring increased estimates of environmental and phenotypic variances, and decreased heritability estimates.
    Type of Medium: Electronic Resource
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