ISSN:
1432-203X
Keywords:
Catharanthus roseus
;
Cell aspect ratio
;
Cell morphology
;
Indole alkaloids
;
Protoclones
;
Vinca
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The relationship between the morphology and indole alkaloid production of Catharanthus roseus cells was investigated. Eleven cell lines were randomly selected from protoplast-derived clones. In each line, most of the cells maintained only one of the two shapes, either spherical or cylindrical. The cell aspect ratio (cell length/width) for most isolates was stable for more than two years of subculture. Cell division patterns of spherical and cylindrical cell isolates were different and patterns of division remained stable in each phenotype and were not considerably affected by auxin or cytokinin levels in the culture media. These observations indicate that cell morphology of our isolates is stable and probably internally determined. Production of the indole alkaloids, ajmalicine and catharanthine was significantly greater when the cell aspect ratio was more than 2.8.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00233292
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