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  • 1
    Electronic Resource
    Electronic Resource
    Cell wall of Thermoanaerobacterium thermosulfurigenes EM1: isolation of its components and attachment of the xylanase XynA (1999)
    Springer
    Archives of microbiology 171 (1999), S. 159-165 
    Details
    ISSN: 1432-072X
    Keywords: Key words S-layer ; Thermophile ; Exocellular ; proteins ; Cell surface
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Thermoanaerobacterium thermosulfurigenes EM1 has a gram-positive type cell wall completely covered by a surface layer (S-layer) with hexagonal lattice symmetry. The components of the cell envelope were isolated, and the S-layer protein was purified and characterized. S-layer monomers assembled in vitro into sheets with the same hexagonal symmetry as in vivo. Monosaccharide analysis revealed that the S-layer is associated with fucose, rhamnose, mannosamine, glucosamine, galactose, and glucose. The N-terminal 31 amino acid residues of the S-layer protein showed significant similarity to SLH (S-layer homology) domains found in S-layer proteins of different bacteria and in the exocellular enzymes pullulanase, polygalacturonate hydrolase, and xylanase of T. thermosulfurigenes EM1. The xylanase from T. thermosulfurigenes EM1 was copurified with the S-layer protein during isolation of cell wall components. Since SLH domains of some structural proteins have been shown to anchor these proteins noncovalently to the cell envelope, we propose a common anchoring mechanism for the S-layer protein and exocellular enzymes via their SLH domains in the peptidoglycan-containing layer of T. thermosulfurigenes EM1.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002030050694
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  • 2
    Electronic Resource
    Electronic Resource
    Helicobacterpylori in Dental Plaque (A Comparison of Different PCR Primer Sets) (1999)
    Springer
    Digestive diseases and sciences 44 (1999), S. 479-484 
    Details
    ISSN: 1573-2568
    Keywords: Helicobacterpylori ; PCR ; DENTAL PLAQUE ; TRANSMISSION
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract This study was designed to compare differentprimer sets for PCR analysis of H. pylori in the sameseries of 40 dental plaque samples. Three pairs ofprimers, HPU1/HPU2, HP1/HP2, and EHC-U/EHC-L, directed to the urease A gene, 16S rRNA gene, or 860-bpDNA of H. pylori, respectively, were used. Our resultsdemonstrate that EHC-L/EHC-U were moRespecific andsensitive for H. pylori added to saliva or dental plaque than HPU1/HPU2 and HP1/HP2. Thedetection rates for H. pylori DNA in dental plaquesamples from randomly selected adult patients from theDental Clinic of the University of Ulm were 26.5% (9/34) for HPU1/HPU2, 78.9% (30/38) for HP1/HP2, and100% (40/40) for EHC-U/EHC-L (P 〈 0.001). Nested PCRusing primers directed to the 860-bp DNA of H. pylorifurThe r confirmed the presence of H. pylori DNA (40/40) in all these samples. Our resultsindicate that primers EHC-U/EHC-L are to be recommendedfor PCR detection of H. pylori in the oralcavity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1026680618122
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    Paper (German National Licenses)
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