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  • 1
    Electronic Resource
    Electronic Resource
    Regulatory volume increase in rat pancreatic β-cells (1997)
    Springer
    Pflügers Archiv 435 (1997), S. 227-230 
    Details
    ISSN: 1432-2013
    Keywords: Key words Pancreas ; Islet cells ; Cell volume ; Na+-2Cl ; -K+ cotransporter ; Na+-H+ exchange ; Cl ; -HCO3 ; exchange
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  This study investigated regulatory volume increase (RVI) in rat pancreatic β-cells. Volume changes in isolated β-cells were measured by a video-imaging method. Cell shrinkage was induced by exposure to solutions made hypertonic by the addition of 100 mM mannitol. In HEPES-buffered solutions, β-cells exhibited an RVI which was almost completely abolished by 10 μM bumetanide. These data indicate that Na+-2Cl–-K+ cotransporters make a major contribution to RVI in β-cells. In HCO3 –-buffered solutions, however, an RVI was observed in the presence of 10 μM bumetanide. This bumetanide-insensitive component of RVI was inhibited by 100 μM amiloride or 100 μM 4,4’-diisothiocyanatostilbene-2,2’-disulphonic acid (DIDS). These data suggest that, in addition to the Na+-2Cl–-K+ cotransporter, functionally coupled Na+-H+ exchangers and Cl–-HCO3 – exchangers may also contribute to RVI in pancreatic β-cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004240050505
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  • 2
    Electronic Resource
    Electronic Resource
    Ca2+-activated K+ channels are involved in regulatory volume decrease in acinar cells isolated from the rat lacrimal gland (1994)
    Springer
    The journal of membrane biology 141 (1994), S. 193-201 
    Details
    ISSN: 1432-1424
    Keywords: Ca2+-activated K+ channels ; Cell volume ; Regulatory volume decrease ; Tetraethylammonium ; Lacrimal acinar cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The volumes of acinar cells isolated from rat lacrimal gland were measured on computer by video-imaging. Cells were found to swell on exposure to hypotonic solutions; they subsequently exhibited a regulatory volume decrease (RVD). RVD was inhibited in the absence of extracellular Ca2+, and by the K+ channel blocker tetraethylammonium chloride (2 mm TEA+). The possible involvement of K+ channels in RVD was further investigated in cell-attached patches. Exposing the cells to a hypotonic solution activated channels with a conductance of 141±6 pS (n=11). These channels were partially blocked by 0.5 mm TEA+, and channel activation was not observed in the absence of extracellular Ca2+. Experiments in the inside-out patch configuration demonstrated that the channels activated by hypotonie stress were “maxi” Ca2+-activated K+ channels. It is concluded that the opening of these channels plays an important role in RVD, by facilitating K+ loss from the cell.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00238253
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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