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  • 1
    ISSN: 1438-2385
    Keywords: Key wordsOlive ; Cell wall ; Processing ; Pitting ; Hemicelluloses
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Olive fruits processed as “Spanish green olives” were sorted into two batches depending on their pitting behaviour: those that broke under punch pressure, and those that were pitted without damage. Cell wall polysaccharides from both batches were isolated. The main changes were lower amounts of carbonate-soluble and 24 % KOH-soluble polysaccharides and a higher proportion of 4% KOH-soluble polysaccharides in broken olives. The carbonate-soluble fraction from broken olives was poorer in homogalacturonans, polysaccharides that could help in increasing texture. The 24% KOH-soluble fraction from the same olive batch was poorer in xylans and xyloglucans, the former being present in a higher proportion in the 4% KOH-soluble fraction and the last in cellulose residue. The new xylans of the 4% KOH-soluble fraction were of high (around 250 kDa) and low (6–40 kDa) molecular weights. Carbonate-soluble and 24% KOH-soluble fractions are very important in maintaining cell wall structure, which is responsible for vegetable product firmness.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1431-4630
    Keywords: Key words Olives ; Cell wall ; Enzymes ; Ethylene ; Storage
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract  The activity of various enzymes (α-D-galactosidase; β-D-galactosidase; α-L-arabinofuranosidase; α-D-mannosidase, β-D-N-acetylglucosaminidase, cellulase and polygalacturonase) associated with the cell wall during olive storage was assayed in order to establish the behaviour of the enzymes as a function of the ripening stage and in relation to the production of ethylene. The effect of exogenous ethylene (100 mg/l for 24 h) was also evaluated. In addition, gaseous emissions of CO2 and ethylene during olive storage were monitored. The results obtained indicate that the high initial CO2 level in the green olive coincides almost exactly in time with the climacteric maxima when the fruit is on the tree. After the rapid decrease in the respiration rate of green olives during storage, the CO2 production rate increases as the stage of maturity advances. The results also indicate that ethylene is not capable of stimulating the activity or synthesis of enzymes in green olives, but can produce such a stimulation in black olives. Furthermore, during the first day of storage there were very marked decreases in enzyme activities. Small variations in the conditions of aerobiosis in post-harvest ripening were shown to have notable effects on the normal metabolism of the fruits.
    Type of Medium: Electronic Resource
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