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  • 1
    Electronic Resource
    Electronic Resource
    Efficient translation of long-lived messengers in extracts from dry pea primary axes (1980)
    Springer
    Planta 150 (1980), S. 286-290 
    Details
    ISSN: 1432-2048
    Keywords: Cell-free translation ; Lectin ; Long-lived mRNA ; Pisum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Extracts prepared from dry pea (Pisum sativum, L; cv oberon) primary axes translate efficiently their endogenous messengers in an in vitro protein synthesizing system. The native long-lived messengers are biologically fully active and direct the synthesis of a whole range of polypeptides with MW ranging up to 130,000. About 0.5% of the total in vitro synthesized polypeptides are recovered in the immunoprecipitate obtained with pea lectin antiserum. Since about one-fourth of the radioactivity in the immunoprecipitate comigrates with authentic pea lectin it is concluded that about 0.1% of the long-lived messengers code for the lectin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00384657
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Sedimentation properties of preformed messenger particles in dry rye embryo extracts (1978)
    Springer
    Planta 140 (1978), S. 171-176 
    Details
    ISSN: 1432-2048
    Keywords: Cell-free translation ; Preformed mRNA ; Rye embryos ; Sedimentation behavior
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Two classes of messenger containing particles can be distinguished in cell-free extracts from dry rye (Secale cereale, L.; var. Celestijner) embryos. A part of the endogenous template activity is associated with large structures, even during in vitro translation. Treatment with detergents results in a partial solubilization of the messenger particles from the large, presumably membranous structures. The sedimentation behavior of the “soluble” mRNP particles (about 75% of the total endogenous template activity) is strongly influenced by the composition of the homogenization medium. At high Mg2+ or Ca2+ concentrations, and at low pH, the soluble mRNP particles form aggregates sedimenting at low centrifugal forces. This peculiar behavior is of partical interest with respect to the preparation of cell-free extracts with low endogenous template activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00384917
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Subunit exchange between lectins from different cereal species (1982)
    Springer
    Planta 154 (1982), S. 568-572 
    Details
    ISSN: 1432-2048
    Keywords: Heteromeric lectin ; Hordeum ; Lectin ; Secale ; Triticale ; Triticum (lectins)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Lectins from Triticum monococcum, Secale cereale (rye), and Hordeum vulgare (barley) can exchange their subunits in vitro and thereby form (intergeneric) heteromeric lectins. An analysis of the isolectin pattern of a Triticale variety revealed that intergeneric heterodimers of wheat and rye lectin subunits are normal constituents of the embryo cells. It appears, therefore, that these different cereal lectins are structurally so closely related that their subunits can not distinguish between identical and nonidentical partners when they associate into dimers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00403002
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Some aspects of the synthesis of long-lived messenger ribonucleoproteins in developing rye embryos (1979)
    Springer
    Planta 144 (1979), S. 485-490 
    Details
    ISSN: 1432-2048
    Keywords: Embryo (mRNA) ; Messenger ribonucleoprotein ; mRNA ; Ribonucleoprotein (messenger) ; Secale ; Seed development
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Untranslated messenger ribonucleoproteins (mRNPs informosomes) are present in developing rye (Secale cereale, L. cv. Celestijner) embryos throughout the last 5 weeks of seed formation. Ribosomal as well as non-ribosomal ribonucleoproteins are formed continuously both in primary axes and scutella until any synthesis of macromolecules stops upon dessication. The content of preformed messengers in the primary axes of precociously harvested rye grains increases as a function of embryo development. This increase of the “template load” of the primary axes results from a continuous accumulation of qualitatively identical mRNPs. Germination experiments demonstrated that at least most of the preformed messengers are not required for the germination process. Their function is discussed in terms of selective adaptation to unfavorable conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00380127
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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