ISSN:
1476-5535
Keywords:
Cellulase
;
Thermophilic
;
Monoclonal antibody
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Summary Cellobiohydrolase II was purified from aMicrobispora bispora culture filtrate and a monoclonal antibody to it was prepared. Screening aM. bispora genomic library inEscherichia coli with this antibody yielded three equivalent clones. Subcloning resulted in greater expression, and activity could be monitored using 4-methylumbelliferylcellobioside. Southern analysis provided evidence that there is a single gene coding for CBH II. The original 22-kb fragment was reduced to 4 kb and subcloned into pUC118/119 resulting in a doubling of expression CBH II. The gene was expressed via its own promoter. The optimal pH (6.5) and the optimal temperature (60°C) of the cloned enzyme are similar to that of the native CBH II.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01583842
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